Millipore’s Magna ChIP2 TM chromatin immunoprecipitation DNA microarray kits are designed to address these challenges and make ChIP-chip analysis easily accessible and affordable with optimized reagents, user-friendly kits, and validated protocols suitable for both the novice and experienced user.
Performance
Using the Magna ChIP2 Kit chromatin associated with HeLa cell RNA polymerase II was immunprecipitated using the Millipore ChIPAb+ anti-RNA polymerase II kit (17-620 and the 05-623 antibody). The isolated DNA was amplified using the reagents provided in the LMPCR module then labeled for one or two color detection according to individual platform requirements. The labeled DNA was hybridized to either the Agilent human 244K promoter array, the Affymetrix® human promoter array or the Nimblegen Human promoter array. The resulting data was analyzed via vendor-related software and the UCSC Genome Browser (http://genome.ucsc.edu)The Magna ChIP2 kit clearly demonstrates RNA polymerase II enrichment at the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene promoter region (Figure A) as expected for an actively transcribed gene. Enrichment at the GAPDH promoter region is detectable independent of the array platform; making the Millipore Magna ChIP2 kit a truly universal system for ChIP chip analysis.
Magna ChIP2 Universal Kits
Optimized reagents and validated protocols for chromatin isolation and amplification, ready for labeling and hybridization to virtually any microarray.| Cat. No. | Description | Materials |
| 17-1000 | Magna ChIP2 Universal Kit | Includes materials sufficient for 6 slides |
| 17-1004 | Magna ChIP2 Universal Quad Kit | Includes materials sufficient for 24 slides |
Magna ChIP2 Human and Mouse Promoter Kits-Just Bring Your Samples
A complete set of reagents for ChIP-chip from start to finish: isolation, amplification, labeling and hybridization, including your choice of either human or mouse Agilent promoter microarrays.
| Cat. No. | Description | Materials |
| 17-1001 | Magna ChIP2 Human Promoter Kit | Includes materials sufficient for 6 slides |
| 17-1002 | Magna ChIP2 Mouse Promoter Kit | Includes materials sufficient for 6 slides |
