High Throughput ChIP HT96 used in a process from the original cells upto the Quantitative PCR and Sequencing
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High Throughput ChIP

The Magna ChIP™ HT96 kit enables up to 96 ChIP reactions in a single plate using as few as 10, 000 cells per well. The kit provides a streamlined protocol compatible with automated liquid handling systems, and a propriety buffer system with a single buffer for sonication, IP, and wash.

ChIP HT96

Use the Magna ChIP™ HT96 kit for excellent sensitivity and lower backgrounds than conventional approaches.

Ordering Information:

Figure A
Figure B
Sonicated chromatin prepared from (A) 10,000 or (B) 100,000 HeLa cells was subjected to chromatin immunoprecipitation using 1 μg of purified mouse IgG (M Cat. No. 12-371B) rabbit IgG (R, Cat. No. 12-370) and specific antibodies (RNA Pol II, Cat. No. 17-620; Phospho-CREB, Cat. No. 17-10131; H3K4Me3, Cat. No.17-614) and the Magna ChIP™ HT96 Kit
The immunoprecipitations shown in (B) were conducted using a Freedom EVO® robotic workstation. Immunoprecipitation of antibody-associated DNA fragments was verified by qPCR using control primers flanking the human GAPDH promoter region
Sonicated chromatin prepared from (A) 10,000 or (B) 100,000 HeLa cells was subjected to chromatin immunoprecipitation using 1 μg of purified mouse IgG (M Cat. No. 12-371B) rabbit IgG (R, Cat. No. 12-370) and specific antibodies (RNA Pol II, Cat. No. 17-620; Phospho-CREB, Cat. No. 17-10131; H3K4Me3, Cat. No.17-614) and the Magna ChIP™ HT96 Kit. The immunoprecipitations shown in (B) were conducted using a Freedom EVO® robotic workstation. Immunoprecipitation of antibody-associated DNA fragments was verified by qPCR using control primers flanking the human GAPDH promoter region. Error bars in (A) represent standard deviation of qPCR triplicates.
Chromatin was derived from sources as indicated, and Mock vs. Specific antibody immunoprecipitations were performed using the multichannel protocol of Magna ChIP™ HT96 on various loci as indicated on ChIPab+ product data sheets
Chromatin was derived from sources as indicated, and Mock vs. Specific antibody immunoprecipitations were performed using the multichannel protocol of Magna ChIP™ HT96 (see product manual for details) on various loci as indicated on ChIPab+ product data sheets.
High Performance using a various ChIP Ab+ Antibodies

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