Enrichment of GAPDH promoter DNA by Magna ChIP Protein A Magnetic Beads vs. a leading competitor’s magnetic beads in identical ChIP assays using ChIPAb+ Acetyl H3 antibody (17-615) and control rabbit IgG. GAPDH is constitutively active and its promoter is expected to be associated with acetylated Histone H3. EMD Millipore’s beads resulted in significantly greater change in PCR threshold values (ΔCT not shown) and greater fold enrichment of GAPDH promoter DNA.
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Magna ChIP2 kits enable genome-wide ChIP analysis using multiple types of microarrays. Comparative results for the Agilent human 244K promoter array (Top), Affymetrix® human promoter array (Middle) and Nimblegen™ human promoter array (Bottom) using the EMD Millipore Magna ChIP2 kit.