What pore size should I select?
Pore size determination depends entirely on your cell type. A quickliterature search will enable you to decide the best pore size for the particular cells you are using. The following chart illustrates pore size choice for cell lines used in our laboratory and by some of our customers for these assays.

Cell Name Cell Type Pore Sizes Typically Used Assays Typically Performed
MDA-MB-231 Invasive breast cancer cell line (human) 5 or 8 µm 5 or 8 µm used in chemotaxis or invasion assay.
MCF7 Non-invasive breast cancer cell line (human) 5 or 8 µm 5 or 8 µm used in chemotaxis or invasion assay.
HT1080 Invasive fibrosarcoma cell line (human) 5 or 8 µm 5 or 8 µm used in chemotaxis or invasion assay.
NIH3T3 Non-invasive fibroblast cell line (mouse) 5 or 8 µm 5 or 8 µm used in chemotaxis or invasion assay.
HUVEC (Human vein umbilical vein endothelial cells) Endothelial cells 3 or 5 or 8 µm 3 or 5 or 8 µm in chemotaxis, invasion, angiogenesis or transendothelial migration assays.
HMVEC/HMEC (Human dermal microvascular) Endothelial cells 5 or 8 µm 5 or 8 µm in chemotaxis, invasion, angiogenesis or transendothelial migration assays.
PMN Polymorphonuclear neutrophils 1 or 3 µm 1 or 3 µm in chemotaxis assays.
Primary Stromal cells 8 µm No information available.
Epithelial cells 3 or 5 µm No information available.
Human coronary artery smooth muscle cells 5 µm No information available.
Hepatic stellate cells Myofibroblast 5 µm No information available.