Biosensors

HeLa cells were transduced with LentiBrite™GFP-LC3 lentiviral particles and were either (A) left in complete media or (B) incubated in EBSS containing a lysosome inhibitor under starvation conditions. The GFP-LC3 displays a diffuse nuclear and cytosolic distribution in fed cells, and a punctate distribution in starved autophagic cells.

Features and Benefits:

• Pre-packaged, ready-to-use, fluorescently-tagged with monomeric GFP & RFP
• Minimum titer (≥3 x 10^8 IFU/mL) per vial
• Higher efficiency transfection as compared to traditional chemical-based and other non-viral-based transfection methods
• Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells
• Non-disruptive towards cellular function
• Validated for fluorescent microscopy and live cell analysis

Live Cell Visualization

• LentiBrite™ GFP-LC3 Lentiviral Biosensor
• LentiBrite™ EB3-GFP Lentiviral Biosensor
• LentiBrite™ RFP-EB3 Lentiviral Biosensor
• LentiBrite™ Histone H2B-RFP Lentiviral Biosensor
• LentiBrite™ Histone H2B-GFP Lentiviral Biosensor

LentiBrite™ GFP-LC3 Lentiviral Biosensor,

LentiBrite™ EB3-GFP Lentiviral Biosensor,

LentiBrite™ RFP-EB3 Lentiviral Biosensor,

LentiBrite™ Histone H2B-RFP Lentiviral Biosensor,

LentiBrite™ Histone H2B-GFP Lentiviral Biosensor,

Higher Efficiency Transfection Compared to Chemical or Non-viral-based Transfection Methods

GFP-Tubulin (HUVEC)   Plasmid Transfection Using Lipofectamine 2000   LentiBrite™ GFP-Tubulin Lentiviral Biosensor Tranfection (Catalogue No. 17-10206)

HUVECs were transfected with GFP-Tubulin plasmids using Lipofectamine 2000 (top row) or LentiBrite™ GFP-Tubulin lentiviral particles (bottom row). The nucleus is stained with DAPI (blue). The LentiBrite lentiviral transfection yields higher efficiency and more uniform expression of GFP-Tubulin versus chemical transfection.

Higher Efficiency Transfection Compared to Baculovirus

MOI = 20 LentiBrite™   Baculovirus Competitor Product

LentiBrite™ lentiviral particles encoding GFP-LC3 (Catalogue No. 17-10193) were adjusted to 1 mL, and equivalent volumes of lentivirus and baculovirus from a competitor product were added to HT1080 cells at an MOI of 20. Lentiviral transfection provides a stronger overall signal of GFP-LC3 compared to baculovirus transfection, when using equivalent amounts of each product.

Ability to Transfect Dividing, Non-dividing, and Difficult-to-transfect Cell Types

LentiBrite™ Paxillin-GFP (Catalogue No. 17-10154) Primary cells (Human Mesenchymal Stem Cells) were plated in chamber slides and transduced with lentiviral particles at an MOI of 5 for 24 hours.		LentiBrite™ RFP-Beta Actin (Catalogue No. 17-10203) Primary cells (HUVECs) were plated in chamber slides and transduced with lentiviral particles at an MOI of 40 for 24 hours.		LentiBrite™ GFP-Vimentin (Catalogue No. 17-10152) Primary cells (Human Mesenchymal Stem Cells) were plated in chamber slide and transduced with lentiviral particles at an MOI of 10 for 24 hours.

LentiBrite™ Lentiviral Biosensor Family