Overview
Cell visualization and microscopy has remained a fundamental tool for investigating cellular behavior and structure, including changes in structural features as well as cellular and sub-cellular dynamics under normal and diseased states. As the technology for microscopy has evolved, the ability to interrogate discrete protein and organelle dynamics has increased and the need for specific visualization tools has grown.

EMD Millipore’s emerging portfolio of biosensors and cell-based assays provide innovative cell visualization and staining tools that complement our suite of fluorescently-conjugated primary and secondary antibodies.
Browse our entire portfolio of conjugated primary and secondary antibodies. Or search our selection of antibodies and assays directly by using the product search tool on the right.

PRODUCT HIGHLIGHT
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 488 Conjugate

Immunocytochemistry Analysis: Positive immunostaining for α-Tubulin in HeLa cells.
Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton and composed of alternating alpha and beta tubulin subunits.

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Milli-Mark™ ChromaPan Neuronal Marker

Immunocytochemistry Analysis: Positive immunostaining of rat cortex primary cells.
Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, EMD Millipore has developed the Milli-Mark™ ChromaPan Neuronal Marker, a complete multi-species neuronal antibody blend that reacts against key somatic, nuclear, dendritic, and axonal proteins distributed across the neuronal architecture.

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Anti-Pan-Neuronal Neurofilament Marker, clone SMI-311

Immunocytochemistry Analysis: Immunostaining of the SMI 311 antibody (green) on rat brain (8um horizontal section). Other structures visualized with anti-GFAP (red, PRB-571C) and Hoechst. Photo courtesy of Molecular Expressions.
Neurofilaments are intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal growth. Neurofilaments are composed of polypeptide chains or subunits that are related structurally to the intermediate filaments of other tissues such as keratin subunits.

This antibody cocktail was selected to provide a specific marker for neurons in tissue sections and cultured cells. In contrast to individual non-phosphoneurofilament antibodies that identify different subsets of neurons, this cocktail is a convenient marker for neurons in general and their differentiation from non-neuronal cells. This antibody cocktail can be used to trace the "inside-out gradient" of neuron production and differentiation in specifically delineating cell bodies and dendrites.

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Anti-Cortactin (p80/85), clone 4F11, Alexa Fluor® 488 Conjugate

Immunocytochemistry Analysis: HeLa cells were stained with anti-Cortactin, clone 4F11, Alexa Fluor® 488 (green) and DAPI (blue).
Cortactin is a monomeric protein that, once stimulated, promotes the polymerization and rearrangement of the actin cytoskeleton. Activated cortactin recruits the Arp2/3 complex proteins to existing actin microfilaments, facilitating and stabilizing nucleation sites for actin branching. Cortactin is important in promoting lamellipodia formation, invadopodia formation, cell migration, and endocytosis.