RNA-binding proteins play a key role in regulating RNA processing, stability, transport, translation, and other post-transcriptional steps.
RNA-binding proteins play a key role in regulating RNA processing, stability, transport, translation, and other post-transcriptional steps.

RNA-binding Proteins (RBP)

RNA-binding proteins (RBPs) play a key role in post-transcriptional regulation of gene expression by binding to RNA along various points and at various times. In the model of mRNA metabolism, this interaction occurs concurrently or immediately after transcription; as different sets of RBPs bind to the introns and exons of pre-mRNA, through splicing, polyadenylation, mRNA stabilization, nuclear transport, subcellular localization and translation. Since these proteins have the potential to affect the manner and rate of protein synthesis, it is crucial to have a reliable method for identifying and characterizing these RBP/RNA interactions.

Role of miRNAs in cell fate determination. ncRNA-binding proteins frequently regulate ncRNA activity, and the differential expression of both ncRNAs and ncRNA binding proteins allows sophisticated patterning, for example, in developing embryos.
Role of miRNAs in cell fate determination. ncRNA-binding proteins frequently regulate ncRNA activity, and the differential expression of both ncRNAs and ncRNA binding proteins allows sophisticated patterning, for example, in developing embryos.

ncRNAs in Post-Transcriptional Gene Regulation

Recent research on post-transcriptional gene regulation has yielded many new discoveries. Most notably, noncoding RNAs (ncRNAs) have been found to regulate many processes listed above. Especially during development and differentiation, ncRNAs provide a finely tuned mechanism for lineage-specific or even cell-specific protein expression. ncRNAs involved in post-transcriptional regulation include snRNAs, snoRNAs, siRNAs, miRNAs, and piRNAs.

Epigenetics and ncRNAs

Most commonly, ncRNAs downregulate protein expression via silencing, epigenetic changes or translational inhibition. However, ncRNAs can upregulate gene expression through certain chromatin modifications. In eukaryotic cells, miRNAs and piRNAs are the most common small RNAs that mediate gene silencing.

RNAi Mechanism

ncRNA-mediated gene silencing (RNAi) is catalyzed by the RNA-induced silencing complex (RISC). RISC is comprised of Argonaute (Ago) proteins and accessory RNAs, and mediates mRNA degradation by complementary small double-stranded RNAs. Specific knockdown of target mRNAs using designed short dsRNA sequences has become a popular genetic tool for analyzing gene function.

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