Cell Culture Media Preparation
The cells used to produce therapeutic proteins require a precise balance of nutrients to support their reproduction and growth. These nutrients are provided in the form of growth media, which must be sterile. For media containing animal derived components, final sterilizing-grade filtration should assure the removal of bacteria and mycoplasma.
Media is typically mixed in bulk and then aseptically transferred to the bioreactor. Prefiltration is used to remove the bulk of particulate and colloidal contaminants from the media in order to extend the service life of the filter train. Prefilters must be sized appropriately to handle batch to batch media variability, as well as ensuring that the sterile media fill in the bioreactor is completed successfully and on time.
Filter Recommendations
Millipore’s Express SHC and SHR filters are recommended for the scale-up and manufacture of tissue culture media for the production of MAbs, vaccines, and other biological therapeutics that require high throughput, flow rates and superior filtration economics.Understanding the Options
- Cell Culture Media Preparation; Bacterial and Mycoplasma Retention
- Cell Culture Media Preparation: Bacterial Retention Only
产品新闻
- Millipore Unveils Single-Use Mobius CellReady 3L Bioreactor at ESACT Meeting
- Millipore Receives Virus Filtration Patents
- Millipore Unveils Mobius® FlexReady Solutions at INTERPHEX 2009
- Millipore Introduces the Mobius® MIX500 Disposable Mixing System - Increased process speed and reduced risk now in a 500L size
- Millipore Expands Its NovAseptic® Mixer Family - The new GMP30000 mixer is ideal for effective mixing in large volumes
- Millipore Introduces New Service Offer for Evaluating Mixing Solutions
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