Microbial Cell Culture Clarification
Microbial fermentation is used to produce products such as therapeutic proteins, antibiotics, hormones, enzymes, amino acids, blood substitutes and alcohol. The product of interest may be expressed by microbial cells or be intracellular (cytoplasmic or periplasmic in the case of bacterial cells).
To obtain the desired product, it must first be separated from the cells and other debris in the fermentation broth. In the case of intracellular products, the cells must be lysed and the lysate must be clarified to remove the cell debris and other contaminants. Depending on the process, additional clarification steps may be needed. Filtration is also used to process fermentation additives such as nutrients and pH adjusters prior to addition into the fermenter.
| Where can recombinant proteins be located? | |
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(1) Secreted into the media (2) Cytoplasm: soluble or aggregated inclusion bodies (3) Periplasm: soluble or aggregated inclusion bodies | |
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Understanding the Options
- Expressed Protein
- Inclusion Body (IB) Purification
- Protein Refold Pool Clarification
- Intracellular Protein (cytoplasmic or periplasmic)
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