Vaccines - Conjugates

Concentration and diafiltration of conjugate prior to reacting with the polysaccharide assures the proper formulation and conditions
Fast, robust process able to accommodate feed fluid variations
Easily scalable from lab to manufacturing scale

Recommended Filter

Filter Characteristics

Low feed flow requirement minimizes pumping, working and hold up volumes.
Higher product recovery and flux
Highly retentive regenerated cellulose membranes offer good permeate flux and withstand process upsets
Robust low binding void-free Ultracel PLC membranes assure consistent processing from batch to batch, have good chemical compatibility and are easy to clean
Complete range of devices for easy scale-up

Recommended Filter	Filter Characteristics
Double-layer Multilayer Durapore 0.45 µm + 0.22 µm Filters Cartridge Filters Opticap Capsule Filters Optiscale Capsule Filters	Sterilizing-grade PVDF 0.22 µm membrane with an additional 0.45 µm prefilter layer all in one pleated device Low-binding membranes assure product yield and sterility 100% Integrity testing ensures quality Double-layer construction provides excellent capacity and protection of downstream equipment Can be integrity tested on-site

Concentration of process fluids and buffer exchange
Will extend the life of expensive chromatography media and improve their speed and efficiency
Fast, robust process able to accommodate feed fluid variations
Wide chemical compatibility with cleaning agents
Easily scalable from lab to manufacturing scale

Recommended Filter

Filter Characteristics

Low feed flow requirement minimizes pumping, working and hold up volumes
Highly retentive regenerated cellulose membranes offer good permeate flux and withstand process upsets
Robust low binding void-free Ultracel PLC membranes assure consistent processing from batch to batch, have good chemical compatibility and are easy to clean
Complete range of devices for easy scale-up
Choose a membrane molecular weight cut-off 3–5X smaller than the biomolecule. Choice should be based on small-scale testing.

ALTERNATIVE: For applications where organic solvents are used, Millipore suggests utilizing a Prostak UF Module with Ultracel membrane.

Recommended Filter

Filter Characteristics

Low protein-binding sterilizing-grade PVDF membrane
Low extractables preserve product purity and yield
High flow rate at low pressure drop for reliable economic processing
100% integrity testing and thousands of applications assure proven performance
Validated for multiple SIP and hot water sanitization cycles
Available in Millipak stacked disc format which allows minimal hold-up volume
Although 0.22 µm membrane is typical for this application, Durapore filters are available in several pore sizes and, in single-layer or multi-layer configurations to accommodate the needs of each user. Choice should be based on small-scale testing.

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