Microbial Cell Culture Clarification: Expressed Protein

Recommended Filter	Filter Characteristics
Depth Filtration Double-layer Millistak+ HC Filters (Grade C0HC or D0HC) Disc Filters Pod Filters	Better suited for small to medium size fermenters up to 1,000 L or larger volumes with low usage rates of less than 10 batches/year Double-layer design enhances loading capacity and filtrate quality Unique configuration allows compression of multiple process steps into one, lowering overall filtration costs Selection of C0HC or D0HC media grade depends on particle size distribution in feed solution. Choice should be based upon small-scale testing.
TFF with Microfiltration Membrane Prostak Open-Channel Modules with 0.22 µm or 0.1 µm Durapore Membrane	Open-channel design ideal for fluids containing high cell densities and solids High flux at low transmembrane pressure allows volume reduction as fluid gets concentrated Robust construction for multiple use Validated for multiple SIP cycles Selection of 0.22 µm or 0.1 µm membrane depends on particle size distribution in feed solution. Choice should be based upon small-scale testing.

Recommended Filter	Filter Characteristics
Single-layer Durapore 0.22 µm PVDF Filters Cartridge Filters Optiseal Cartridge Filters Opticap Capsule Filters Optiseal Capsule Fitlers	Sterilizing-grade PVDF membrane won’t bind valuable protein 100% Integrity tested to ensure proven performance Can be integrity testable on-site Although 0.22 µm is typical for this application, Durapore filters are available in several pore sizes and, in single-layer or multi-layer configurations to accommodate the needs of each user, the degree of protection required for the downstream process and the plugging characteristics of the fluid. Choice should be based on small-scale testing.

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