Luminex 200™ Multiplexing Instrument

• Luminex 200™ Instrument Specifications

  Accuracy and Precision Sample uptake volume ±5% Classification of microspheres >80% Misclassification of microspheres ≤ 2%. May vary by xMAP microsphere product lines. Refer to the specific product information sheet for further details. Temperature control 0°C to +2°C of target Internal sample carryover <0.9% Soluble background fluorescence emissions at 575 nm automatically subtracted from fluorescence intensity values		Sensitivity Detect 1000 fluorochromes phycoerythrin (PE) per xMAP microsphere Reporter channel dynamic range: 3.5 decades of detection
  Optics Reporter laser: 532 nm, nominal output 10 - 15 mW, maximum 500 mW, frequency-doubled diode; mode of operation, continuous wave (CW) Classification laser: 635 nm, 9.1 ± 6%, maximum output 25 mW, diode; mode of operation, continuous wave (CW) Reporter detector: Photomultiplier tube, detection bandwidth of 565 – 585 nm Classification detector and doublet discriminator: Avalanche photo diodes with temperature compensation		Fluidics Sheath flow rate: 90 µL ± 5 µL/second Cuvette: 200 micron square flow channel Sample injection rate: 1 µL/second Sample update volume: 20 – 200 µL
  Setup Installation < 4 hours System calibration < 10 minutes System warm up 30 minutes. Systems that remain inactive for at least four hours will require a warm-up to restart the lasers. After acquiring sample, running system calibrators, running system controls and warming up the instrument, the system resets the four-hour internal clock.		Capacity The specifications below reflect minimum capacity values: Analyze multiple 96-well plates per batch Analyze multiple assay templates per plate Automatic sampling from a 96-well plate. The following microtiter plates are compatible with the Luminex XY Platform plate holder: flatbottom, conical, round, filter bottom, half plates [overall height no more than 0.75” (19mm)], any color. Sheath container and waste container hold enough volume to run up to two 96-well plates between refills. Distinguish a minimum of 1 to a maximum of 100 unique xMAP microsphere sets in a single sample Detect and distinguish surface reporter fluorescence emissions at 575 nm on the surface of 1-100 unique xMAP microsphere sets in a single sample
  General Operating temperature: 15°C to 30°C (59°F to 86°F) Humidity: 20% to 80%, noncondensing Altitude: Operation up to 2400 m (7874 ft.) above mean sea level Physical dimensions: 43 cm (17 inches) W x 50.5 cm (20 inches) D x 24.5 cm (9.5 inches) H Weight: 25 kg (60 lbs.) UL Installation Category II, as defined in Annex J of the UL 61010A-1 UL Pollution Degree 2, as defined in Section 3.7.3.2 of UL 61010A-1 Luminex 200 analyzer, input voltage range: 100 - 120 V~ ±10%, 1.4 Amp, and 200 - 240 V~ ±10%, 0.8 Amp, 47 – 63 Hz		PC Specifications Intel Pentium IV or equivalent class PC, minimum speed 933 MHz Main memory: minimum of 256 MB Hard disk drive: 10 GB minimum storage capacity Communication/Ports: minimum of one parallel port, 2 RS-232 compatible serial ports, and one USB version 1.1 compatible high speed port Read, write and removable media: CDR/W drive Operating system: Microsoft Windows XP Pro SP2 CE marked and UL listed Two-button mouse or equivalent

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