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RNA Binding Protein Immunoprecipitation:
Representative lot data.
RIP Lysate prepared from HeLa cells (2 X 107 cell equiv...
Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from HeLa cells (2 X 106 cell equivalents per IP...
RIPAb+ SNRNP70
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Ca, M, R, B | RIP, IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
RIPAb+ SNRNP70
Trade Name:
Upstate (Millipore)
Product Overview:
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction.
The RIPAb+ SNRNP70 set includes the SNRNP70 antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human cDNA of U1snRNA. The SNRNP70 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of SNRNP70-associated RNA.
The RIPAb+ SNRNP70 set includes the SNRNP70 antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human cDNA of U1snRNA. The SNRNP70 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of SNRNP70-associated RNA.
Specificity:
SNRNP70
Molecular Weight:
SNRNP70, observed at ~60 & 48 kDa (Predicted molecular weight ~52 kDa).
Immunogen:
The SNRNP70 purified antibody is made against a synthetic peptide corresponding to a region of human SNRNP70.
Species Reactivity:
- Human
- Canine
- Mouse
- Rat
- Bovine
Application Notes:
Immunoprecipitation from RIP lysate:
RIP lysate from HeLa cells (2 X 106 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-SNRNP70 antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SNRNP70 (1.0 μg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures).
RIP lysate from HeLa cells (2 X 106 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-SNRNP70 antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SNRNP70 (1.0 μg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures).
Control:
Included negative control rabbit IgG antibody and control primers specific for U1snRNA.
Quality Assurance:
RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 107 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, or Anti-SNRNP70 antibody and the Magna RIP A Kit (Cat. # 17-700).
Successful immunoprecipitation of SNRNP70-associated RNA was verified by qPCR using RIP Primers U1 snRNA (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
RIP Lysate prepared from HeLa cells (2 X 107 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, or Anti-SNRNP70 antibody and the Magna RIP A Kit (Cat. # 17-700).
Successful immunoprecipitation of SNRNP70-associated RNA was verified by qPCR using RIP Primers U1 snRNA (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
Purification Method:
Purified
Presentation:
Anti-SNRNP70 (Rabbit Polyclonal IgG)
One vial containing 50 μg of antibody lyophilized from PBS buffer with 2% sucrose. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL. For longer periods of storage store at -20°C.
Normal Rabbit IgG
One vial containing 125 μg of purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
RIP Primers, U1snRNA
One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C.
FOR: GGG AGA TAC CAT GAT CAC GAA GGT
REV: CCA CAA ATT ATG CAG TCG AGT TTC CC
One vial containing 50 μg of antibody lyophilized from PBS buffer with 2% sucrose. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL. For longer periods of storage store at -20°C.
Normal Rabbit IgG
One vial containing 125 μg of purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
RIP Primers, U1snRNA
One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C.
FOR: GGG AGA TAC CAT GAT CAC GAA GGT
REV: CCA CAA ATT ATG CAG TCG AGT TTC CC
Storage Conditions:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- SNRNP70
- U1SNRNP
- RNPU1Z
- RPU1
- U1AP1
- U1SNRNP70
- SNRPN
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- RNA Binding Protein Immunoprecipitation (RIP)
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.
SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated FUSIP1 and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2.
SUBCELLULAR LOCATION: Nucleus.
POST-TRANSLATIONAL MODIFICATION: The N-terminus is blocked.
Extensively phosphorylated on serine residues in the C-terminal region.
INVOLVEMENT IN DISEASE: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.
SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.
SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated FUSIP1 and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2.
SUBCELLULAR LOCATION: Nucleus.
POST-TRANSLATIONAL MODIFICATION: The N-terminus is blocked.
Extensively phosphorylated on serine residues in the C-terminal region.
INVOLVEMENT IN DISEASE: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.
SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.
Qty/Pk:
10 assays
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Packaging:
10 assays per set. Recommended use: ~5 μg antibody per RIP (dependent upon biological context).
Product Name:
RIPAb+ SNRNP70
Antibody Type:
Polyclonal Antibody