Product Images
Western Blot Analysis:
Mouse brain and rat brain (lanes 1 and 2, respectively) lysates were resolved by electrophoresis, transferred to PVDF and ...
Immunoprecipitation:
PTEN was immunoprecipitated from NIH/3T3 cells using anti-PTEN, clone 6H2.1 (lanes 2 and 4) and was resolved via SDS-PAGE a...
 lysates were resolved by electrophoresis, transferred to PVDF and probed with anti-PTEN, clone 6H2.1 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates PTEN<br />'))
 and was resolved via SDS-PAGE along side NIH/3T3 lysate (lanes 1 and 3) and transferred to PVDF. The blots were then probed with either anti-PTEN, clone 6H2.1 (lanes 1 and 2) or anti-phosphoserine, clone 4A4 (Cat. No. 05-1000). <br />Arrow indicates PTEN.<br />'))
:<br />Optimal Staining with EDTA Epitope Retrieval:<br />IH staining of paraffin embedded In Situ Ductal Carcinoma with anti-PTEN, clone 6H2.1. Tissue was pretreated with EDTA pH 8, antigen retrieval. Antibody diluted to 1:50, IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as strong nuclear staining signal of malignant in situ tumor cells (TMA spot). 40X Mag.<br />'))
Anti-PTEN, clone 6H2.1
Recommended Replacement for: 07-016| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IP, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-PTEN Antibody, clone 6H2.1 | 04-035
Replaces:
07-016
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
recognizes PTEN
Molecular Weight:
55 kDa
Epitope:
C-terminal region
Immunogen:
C-terminal 100 amino acids of PTEN
Clone:
6H2.1
Isotype:
IgG
Background Information:
The protein and lipid phosphatase PTEN (phosphatase and tensin homolog deleted on chromosome ten) has been suggested to play a role in tumorgenesis. PTEN is a tumor suppressor whose loss permits constitutive signaling through the PI3K pathway and is sufficient to cause cancer. In normal cells, in conjunction with FOXO1A and PTEN function together to restrain cell growth. PTEN metabolizes the product of PI3K phosphorylation of PI(4,5)P2 following receptor activation, creating PI(3,4,5)P3. In cells lacking PTEN, there are elevated levels of PI(3,4,5)P3, which acts as a potent second messenger promoting oncogenesis. Restoration of PTEN function is considered a novel approach to cancer therapy. Overexpression of PTEN inhibits cell migration, and expression of antisense PTEN mRNA enhances migration.
Species Reactivity:
- Human
- Mouse
- Rat
Quality Assurance:
routinely evaluated on HeLa cell lysate
Purification Method:
Protein G purfied
Presentation:
Protein G purified mouse IgG in storage buffer containing PBS with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PTEN
- MMAC1
- TEP1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunoprecipitation
- Immunohistochemistry (Paraffin)
Brand Family:
Upstate
Product Name:
Anti-PTEN, clone 6H2.1
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse