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Immunocytochemistry Analysis:
Representative lot data.
Immunofluorescent staining of PC12 cells using Anti-Neurofilament-66, clone EP676Y.
Immunohistochemistry (Paraffin) Analysis:
Representative lot data.
Immunohistochemical staining of paraffin-embedded human brain using Anti-...
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Anti-Neurofilament-66, clone EP676Y, Rabbit Monoclonal
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH(P), IC, IP, FC | Rabbit | Unpurified | Monoclonal Antibody |
Description:
Anti-Neurofilament-66, clone EP676Y, Rabbit Monoclonal
Specificity:
This antibody recognizes Neurofilament-66 at and around the tail domain.
Molecular Weight:
~66 kDa
Epitope:
Tail Domain
Immunogen:
Synthetic peptide corresponding to residues in the tail domain of human Neurofilament-66.
Clone:
EP676Y
Isotype:
IgG
Background Information:
Neurofilament-66, also known as α-internexin, is a member of the IF family. It is found in developing neuroblasts and in the CNS of adults. Neurofilament-66 is expressed in the adult brain at lower levels than other neurofilament proteins.
Species Reactivity:
- Human
- Rat
Species Reactivity Note:
Human and rat.
Application Notes:
Immunohistochemistry Analysis: A 1:250-500 dilution from a previous lot detected Neurofilament-66 in human brain tissue but not in human colon, kidney or uterus.
Immunocytochemistry Analysis: A 1:250 - 500 dilution from a previous lot detected Neurofilament-66 in PC12 cells.
Flow Cytometry: A 1:25 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Immunocytochemistry Analysis: A 1:250 - 500 dilution from a previous lot detected Neurofilament-66 in PC12 cells.
Flow Cytometry: A 1:25 dilution of a previous lot was used in flow cytometry.
Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.
Control:
SHSY5Y cell lysate
Quality Assurance:
Evaluated by Western Blot on SHSY5Y cell lysates.
Western Blot Analysis: 1:5,000-10,000 dilution of this antibody was used to detect Neurofilament-66 in SHSY5Y cell lysate.
Western Blot Analysis: 1:5,000-10,000 dilution of this antibody was used to detect Neurofilament-66 in SHSY5Y cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- Alpha-Inx
- FLJ18662
- FLJ57501
- MGC12702
- NEF5
- NF-66
- Neurofilament-66
- OTTHUMP00000020403
- TXBP-1
Alternate Names:
- 66 kDa neurofilament protein
- internexin neuronal intermediate filament protein, alpha
- neurofilament 5 (66kD)
- neurofilament-66, tax-binding protein
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
- Immunocytochemistry
- Immunoprecipitation
- Flow Cytometry
Entrez Gene Summary:
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons.
UniProt Summary:
FUNCTION: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
TISSUE SPECIFICITY: Found predominantly in adult CNS.
DEVELOPMENTAL STAGE: Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
PTM: O-glycosylated By similarity.
Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the intermediate filament family.
TISSUE SPECIFICITY: Found predominantly in adult CNS.
DEVELOPMENTAL STAGE: Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
PTM: O-glycosylated By similarity.
Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the intermediate filament family.
Protein/Isoform Description:
Intermediate Filaments (IFs) are neuronal cytoskeletal structures that are at least partly composed of the neurofilament (NF) triplet proteins, the light (NF-L), mid-sized (NF-M), and heavy (NF-H) neurofilament subunits. These proteins are encoded by separate genes and migrate at ~ 68, 150, and 200 kDa, respectively, on SDS-PAGE. NF proteins have an unusually high content of phosphoserine residues, located in lysine-serine-proline (KSP) repeat regions; NF-H subunits may contain as many as 40-50 repeats in tandem arrays; NF-M subunits contain variable numbers of KSP repeats.
Product Name:
Anti-Neurofilament-66, clone EP676Y, Rabbit Monoclonal
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Unpurified
Host:
Rabbit