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Flow Cytometry Analysis:
Representative lot data.
Flow cytometric analysis of permeabilized Jurkat cells using anti-CD45 (red) and a rabbit...
Western Blot Analysis:
Representative lot data.
Jurkat cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed ...
Anti-CD45 (C-term), clone EP322Y, Rabbit Monoclonal
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, FC | Rabbit | Unpurified | Monoclonal Antibody |
Description:
Anti-CD45 (C-term), clone EP322Y, Rabbit Monoclonal
Specificity:
This antibody recognizes CD45 at and around the C-terminus.
Molecular Weight:
~180 kDa
Epitope:
C-terminus
Immunogen:
Synthetic peptide corresponding to residues in the C-terminal domain of human CD45.
Clone:
EP322Y
Isotype:
IgG
Background Information:
CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
Species Reactivity:
Human
Species Reactivity Note:
Human
Application Notes:
Flow Cytometry Analysis:
Flow cytometric analysis of permeabilized Jurkat cells using anti-CD45.
Flow cytometric analysis of permeabilized Jurkat cells using anti-CD45.
Control:
Jurkat lysates
Quality Assurance:
Evaluated by Western Blotting on Jurkat cell lysates.
Western Blotting Analysis:
1:5,000 - 10,000 dilution of this antibody was used to detect CD45 in Jurkat cell lysate.
Western Blotting Analysis:
1:5,000 - 10,000 dilution of this antibody was used to detect CD45 in Jurkat cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- B220
- CD45
- CD45R
- EC 3.1.3.48
- GP180
- L-CA
- LCA
- LY5
- T200
- glycoprotein
Alternate Names:
- CD45 antigen
- T200 glycoprotein
- T200 leukocyte common antigen
- leukocyte-common antigen
- protein tyrosine phosphatase, receptor type, C
- protein tyrosine phosphatase, receptor type, c polypeptide
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Flow Cytometry
Entrez Gene Summary:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq].
UniProt Summary:
FUNCTION: Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN. Ref.6 Ref.8
Catalytic activity Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
SUBUNIT STRUCTURE: Binds GANAB and PRKCSH By similarity. Interacts with SKAP1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: The first PTPase domain interacts with SKAP1.
PTM: Heavily N- and O-glycosylated.
DISEASE: Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-B+NK+SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Ref.14
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. Ref.13
SEQUENCE SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
Catalytic activity Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
SUBUNIT STRUCTURE: Binds GANAB and PRKCSH By similarity. Interacts with SKAP1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: The first PTPase domain interacts with SKAP1.
PTM: Heavily N- and O-glycosylated.
DISEASE: Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-B+NK+SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Ref.14
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. Ref.13
SEQUENCE SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains.
Protein/Isoform Description:
CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
Product Name:
Anti-CD45 (C-term), clone EP322Y, Rabbit Monoclonal
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μL
Format:
Unpurified
Host:
Rabbit