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Immunohistochemistry (Paraffin) Analysis:
Representative lot data.
Immunohistochemical staining of paraffin-embedded human tonsils using Ant...
Western Blot Analysis:
Representative lot data.
HeLa, HepG2, and Jurkat cell lysates were resolved by electrophoresis, transferred to PVDF m...
Anti-PTP1B, clone EP1841Y, Rabbit Monoclonal
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, FC, IH(P), IC | Rabbit | Unpurified | Monoclonal Antibody |
Description:
Anti-PTP1B, clone EP1841Y, Rabbit Monoclonal
Specificity:
This antibody detects both phosphorylated and nonphosphorylated PTP1B.
Molecular Weight:
~50 kDa
Epitope:
Ser386
Immunogen:
Synthetic peptide corresponding to residues surrounding Ser386 of human PTP1B.
Clone:
EP1841Y
Isotype:
IgG
Background Information:
PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and is a drug discovery target in the fields of diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.
Species Reactivity:
Human
Species Reactivity Note:
Human
Application Notes:
Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected PTP1B in human tonsil tissue.
Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC.
Flow Cytometry: A 1:20 dilution of a previous lot was used in flow cytometry.
Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC.
Flow Cytometry: A 1:20 dilution of a previous lot was used in flow cytometry.
Control:
HeLa, HepG2, and Jurkat cell lysates
Quality Assurance:
Evaluated by Western Blot on HeLa, HepG2, and Jurkat cell lysates.
Western Blot Analysis: A 1:1,000-2,000 dilution of this antibody was used to detect PTP1B in HeLa, HepG2, and Jurkat cell lysates.
Western Blot Analysis: A 1:1,000-2,000 dilution of this antibody was used to detect PTP1B in HeLa, HepG2, and Jurkat cell lysates.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- EC 3.1.3.48
- OTTHUMP00000031266
- PTP-1B
- PTP1B
Alternate Names:
- Protein-tyrosine phosphatase 1B
- protein tyrosine phosphatase 1B
- protein tyrosine phosphatase, non-receptor type 1
- protein tyrosine phosphatase, placental
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Flow Cytometry
- Immunohistochemistry (Paraffin)
- Immunocytochemistry
Entrez Gene Summary:
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
UniProt Summary:
FUNCTION: May play an important role in CKII- and p60c-src-induced signal transduction cascades By similarity.
CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
Product Name:
Anti-PTP1B, clone EP1841Y, Rabbit Monoclonal
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Unpurified
Host:
Rabbit