Ordering Information
- : 04-1481
- : 100 μg
- : 1 mg/mL
Product Images
Western Blot (Snap ID) Analysis:
Representative lot data.
HeLa lysate was resolved by electrophoresis, transferred to PVDF membranes and pro...
Western Blot analysis:
Representative lot data.
HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed w...
 Analysis:<br />Representative lot data.<br />HeLa lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-RPA2 p34, clone RPA20, 1-46 (1 µg/mL). <br />Proteins were visualized using a goat anti-mouse IgG conjugated to HRP and a chemiluminescence detection system. <br />Arrow indicates RPA2 (~32 kDa).'))
.<br />Proteins were visualized using a goat anti-mouse IgG conjugated to HRP and a chemiluminescence detection system. <br />Arrow indicates RPA2 (~32 kDa).'))
: Flow Cytometry analysis using HeLa cells, stained with anti-RPA2 p34 (green), or isotype control antibody (grey). '))
Anti-RPA2 p34 Antibody, clone RPA20 1-46
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-RPA2 p34 Antibody, clone RPA20 1-46 | 04-1481
Promotional Text:
Specificity:
This antibody recognizes RPA2.
Molecular Weight:
~32 kDa
Epitope:
Unknown
Immunogen:
His-tagged recombinant protein corresponding to human RPA2.
Clone:
RPA20, 1-46
Isotype:
IgG1κ
Background Information:
RPA is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits, RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa), and plays multiple roles in DNA metabolism. RPA is required for DNA replication initiation, as well as replication elongation. At the onset of DNA replication, RPA is loaded onto chromatin after the binding of Cdc45 to origins. RPA is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After DNA replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination.The 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK. Alternate names for RPA32 include replication protein A 32 kDa subunit, RP-A, RF-A, replication factor-A protein 2, p32, p34, RPA2, REPA2, and RPA32.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Proven to react with human and mouse.
Application Notes:
Western Blot (SNAP ID) Analysis: 0.5 µg/mL from a previous lot detected RPA2 on 10 µg of NIH/3T3 cell lysate.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected RPA2 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected RPA2 on 10 µg of HeLa cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- RPA2
- REPA2
- RPA32
- RPA34
Alternate Names:
- RF-A protein 2
- RP-A p32
- RP-A p34
- Replication factor A protein 2
- replication protein A2 (32kD)
- replication protein A2, 32kDa
- Replication protein A 32kDa subunit
- RF-A, replication factor-A protein 2
- p32
- p34
- RPA2
- REPA2
- RPA32.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.
SUBCELLULAR LOCATION: Nucleus. Note: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.
SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.
SUBCELLULAR LOCATION: Nucleus. Note: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.
Product Name:
Anti-RPA2 p34, clone RPA20 1-46
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse