Ordering Information
- : 04-1523
- : 100 μg
- : 1 mg/mL
Product Images
Western Blot (Snap ID) Analysis:
Representative lot data.
HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes an...
Western Blot analysis:
Representative lot data.
HeLa and Hek293 cell lysates were resolved by electrophoresis, transferred to PVDF membranes...
 Analysis:<br />Representative lot data.<br />HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with anti-CAF p60 diluted to 1 ug/mL.. <br />Proteins were visualized using a goat anti-mouse secondaryantibody conjugated to HRP using a chemiluminescence detection system. <br />Arrow indicates CAF p60 (~61kDa).'))
.'))
: Flow Cytometry analysis using HeLa cells, stained with CAF1 p60 (green), or isotype control antibody (grey). '))
Anti-CAF1 p60 Antibody, clone SS-53, 1-124
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IP | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-CAF1 p60 Antibody, clone SS-53, 1-124 | 04-1523
Promotional Text:
Specificity:
Antibody recognizes CAF p60.
Molecular Weight:
61 kDa
Epitope:
Unknown
Immunogen:
His-tagged human recombinant protein corresponding to CAF p60.
Clone:
clone SS-53, 1-124
Isotype:
IgG1
Background Information:
Chromatin assembly factor (CAF), is a heterotrimeric complex that belongs to the histone chaperone family. It is found in yeast, animals and plants, and facilitates the assembly of chromatin during DNA replication and repair. There are three CAF subunits: p48, p60, and p150. The p60 subunit interacts with the histone chaperone anti-silencing factor 1. Mutations in CAF p60 resulted in apoptosis in dividing human cells.
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Application Notes:
Western Blot (SNAP ID) Analysis: 1 µg/ml from a representative lot detected CAF on 10 µg of HeLa cell lysate.
Control:
HeLa and Hek293 cell lysates
Quality Assurance:
Evaluated by Western Blot in HeLa and Hek293 cell lysates.
Western Blot Analysis: 0.5 µg/ml of this antibody detected CAF on 10 µg of HeLa and Hek293 cell lysates.
Western Blot Analysis: 0.5 µg/ml of this antibody detected CAF on 10 µg of HeLa and Hek293 cell lysates.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CHAF1B
- CAF-1
- CAF-IP60
- CAF-Ip60
- CAF1
- CAF1A
- CAF1P60
- MPHOSPH7
- MPP7
Alternate Names:
- CAF-1 subunit B
- CAF-I 60 kDa subunit
- Chromatin assembly factor I p60 subunit
- M-phase phosphoprotein 7
- chromatin assembly factor 1 subunit B
- chromatin assembly factor 1, subunit B (p60)
- human chromatin assembly factor-I p60 subunit
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunoprecipitation
UniProt Summary:
FUNCTION: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci By similarity. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
SUBUNIT STRUCTURE: Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.
SUBCELLULAR LOCATION: Nucleus. Note: DNA replication foci.
DEVELOPMENTAL STAGE: Active complex is found in G1, S and G2 phases.
DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the CHAF1A family.
SUBUNIT STRUCTURE: Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.
SUBCELLULAR LOCATION: Nucleus. Note: DNA replication foci.
DEVELOPMENTAL STAGE: Active complex is found in G1, S and G2 phases.
DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the CHAF1A family.
Product Name:
Anti-CAF1 p60, clone SS-53, 1-124
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse