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Ordering Information

  • : 04-230
  • : 100 μL
  • Product Family Information

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Immunofluorescent analysis of HeLa cells using anti-Histone Deacetylase 2 (C-term) antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Histone Deacetylase 2 (C-term) antibody.

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Anti-HDAC3, clone Y415, rabbit monoclonal Antibody (04-230)

Species Reactivity Key Applications Host Format Antibody Type
H, M, R ICC, IHC, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-HDAC3 Antibody, clone Y415, rabbit monoclonal
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes Histone Deacetylase 3 at the C-terminus.
Molecular Weight:
49 kDa
Epitope:
C-terminus
Immunogen:
KLH conjugated to a synthetic peptide from the C-terminal region of human Histone Deacetylase 3.
Clone:
Isotype:
Background Information:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone Deacetylase 3 (HDAC3) belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. HDAC3 is regarded as a potential tumor suppressor gene that also plays critical roles in S phase progression and DNA damage control.
Species Reactivity:
  • Human
  • Mouse
  • Rat
Species Reactivity Note:
Demonstrated to react with human, mouse and rat HDAC3.
Application Notes:
Immunoprecipitation:
1:50 dilution from a previous lot was used for immunoprecipitation.
Control:
K562 whole cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on K562 whole cell lysate.

Western Blot Analysis:
A 1:10,000 dilution of this lot was used to detect Histone Deacetylase 3 in K562 whole cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • EC 3.5.1.98
  • HD3
  • RPD3
  • RPD3-2
  • SMAP45
Alternate Names:
histone deacetylase 3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Entrez Gene Summary:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events.
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq].
UniProt Summary:
FUNCTION: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor.

Catalytic activity Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

SUBUNIT: Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1 and SRY. Interacts with BTBD14B. Interacts with GLIS2 By similarity. Interacts with CBFA2T3.

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY: Widely expressed.

PTM: Sumoylated in vitro. Ref.14

SIMILARITY: Belongs to the histone deacetylase family. Type 1 subfamily.

Brand Family:
Upstate
Product Name:
Anti-HDAC3, clone Y415, rabbit monoclonal Antibody (04-230)
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μL
Format:
Culture Supernatant
Host:
Rabbit
Applications:
Use Anti-HDAC3 Antibody, clone Y415 (Rabbit Monoclonal Antibody) validated in ICC, IHC, IHC(P), IP, WB to detect HDAC3 also known as histone deacetylase 3.

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