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Immunoblot Analysis Lysates from A431 cells was resolved by electrophoresis, transferred to PVDF and probed with anti- Acetyl CoA Carboxylase (...
Immunohistochemistry A 1:50 dilution of this lot detected Acetyl CoA Carboxylase in paraffin-embedded human liver using the anti- Acetyl CoA Ca...
Anti-Acetyl CoA Carboxylase 1, Rabbit mAb
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | Rabbit | Purified | Monoclonal Antibody |
Description:
Anti-Acetyl CoA Carboxylase 1, Rabbit mAb
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Acetyl CoA Carboxylase 1
Molecular Weight:
265 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to human Acetyl CoA Carboxylase 1
Species Reactivity:
- Human
- Mouse
- Rat
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 0.09 M NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
Storage Conditions:
Stable for 2 years at -20°C from date of shipment.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ACACA
- ACC-alpha
- ACCA
- ACC
- ACC1
- ACAC
Alternate Names:
ACACA, ACAC, ACC, ACC1, ACCA, acetyl-Coenzyme A carboxylase alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: Q13085 # Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
COFACTOR: Biotin. & Binds 2 manganese ions per subunit.
SIZE: 2346 amino acids; 265554 Da
SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.
SIMILARITY: SwissProt: Q13085 ## Contains 1 ATP-grasp domain. & Contains 1 biotin carboxylation domain. & Contains 1 biotinyl-binding domain. & Contains 1 carboxyltransferase domain.
COFACTOR: Biotin. & Binds 2 manganese ions per subunit.
SIZE: 2346 amino acids; 265554 Da
SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.
SIMILARITY: SwissProt: Q13085 ## Contains 1 ATP-grasp domain. & Contains 1 biotin carboxylation domain. & Contains 1 biotinyl-binding domain. & Contains 1 carboxyltransferase domain.
Qty/Pk:
100 µL
Brand Family:
Upstate
Format:
Purified
Host:
Rabbit
Product Name:
Anti-Acetyl CoA Carboxylase 1, Rabbit mAb
Antibody Type:
Monoclonal Antibody