Ordering Information
- : 05-1050X
- : 30 μL
Product Images
Lysates from various cells with various treatments to stimulated varying tyrosine phosphorylations on multiple substrates were resolved in parallel by...
A431 cells either untreated (left) or EGF-treated (right). Cells were stained with 4G10® Platinum (Anti-phosphotyrosine, green) and DAPI (nuclei, blue...
see more >, PY20 (Cat. No. 05-947), or 4G10® Platinum (Cat. No. 05-1050). The various blots show the advantages of the anti-phosphotyrosine monoclonal cocktail, 4G10® Platinum.')){kind=spacer}
 or EGF-treated (right). Cells were stained with 4G10® Platinum (Anti-phosphotyrosine, green) and DAPI (nuclei, blue). Cells were visualized by confocal immunofluorescent microscope.'))
 staining of various cancer tissue samples.'))
<br />EGF-stimulated A431 cell lysate (Cat. No.12-302) was resolved by SDS-PAGE and transferred to PVDF membrane. PCDF was probed with 4G10® Platinum, anti-phosphotyrosine (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.')){kind=spacer}
Anti-Phosphotyrosine, 4G10® Platinum
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB, IF, FC, IHC, IP, ICC, ELISA | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody, 4G10® Platinum | 05-1050X
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Tyrosine-phosphorylated proteins from all species
Molecular Weight:
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Immunogen:
4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein.
Modifications:
Phosphorylation
Clone:
4G10
Isotype:
IgG2b
Background Information:
The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
Species Reactivity:
All
Control:
Suggested Positive Antigen Control: Cat.# 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at –20ºC. The lysate is stable for 6 months at –20ºC.
Quality Assurance:
Routinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells
Purification Method:
Protein G-Sepharose™ chromatography
Presentation:
A proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2bκ) and PY20 (IgG2b) in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.
Storage Conditions:
2 years at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
NOTE: DO NOT FREEZE.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunofluorescence
- Flow Cytometry
- Immunohistochemistry
- Immunoprecipitation
- Immunocytochemistry
- ELISA
Brand Family:
Upstate
Product Name:
Anti-Phosphotyrosine, 4G10® Platinum
Antibody Type:
Monoclonal Antibody
Qty/Pk:
30 μL
Format:
Purified
Host:
Mouse