Anti-ILK, clone 6G6.3

Anti-ILK, clone 6G6.3

05-575

Upstate (Millipore)

Recognizes ILK

51 kDa

Full length recombinant GST-tagged human ILK.

6G6.3

IgG3κ

ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion/migration. The kinase activity appears to be reduced following Integrin activation, and overexpression of p59ILK inhibits cellular adhesion to Integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).

• Mouse
• Human
• Rat

Not recommended for IP

Routinely evaluated by western blot on HEK293 cell lysate.

Protein G purfied

Protein G purified mouse monoclonal in storage buffer containing 0.1M Tris-Glycine, pH7.4, 15 mM NaCl, and 0.05% NaN3.

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.

Q13418

NP_004508.1

• ILK
• p59ILK
• ILK-1
• P59
• ILK1
• ILK-2
• ILK2
• DKFZp686F1765

• Integrin-Linked Kinase
• p59
• DKFZp686F1765
• p59ILK

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Western Blotting

FUNCTION: SwissProt: Q13418 # Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
SIZE: 452 amino acids; 51419 Da
SUBUNIT: Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts with LIMS1, LIMS2, parvins and probably TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side.
TISSUE SPECIFICITY: Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.DOMAIN:SwissProt: Q13418 A PH-like domain is involved in phosphatidylinositol phosphate binding.
PTM: Autophosphorylated on serine residues.
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 3 ANK repeats. & Contains 1 protein kinase domain.

100 μg

Upstate

Purified

Mouse

Anti-ILK, clone 6G6.3

1 mg/mL

Monoclonal Antibody