Ordering Information
- : 05-1076
- : 100 µg
- : 1 mg/mL
Product Images
Immunocytochemistry Analysis: Representative image from a previous lot.
Confocal fluorescent analysis of C2C12, A431 and HeLa cells using anti-c...
Western Blot Analysis:
Representative lot data.
NIH/3T3 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with ant...
. <br />Actin filaments have been labeled with Alexa Fluor®488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive nuclear staining.<br /><br />'))
. <br />Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. <br /><br />Arrow indicates c-Jun (~36-43 kDa).<br />'))
: Flow Cytometry analysis using NIH/3T3 cells, stained with anti-c-Jun (green), or isotype control antibody (grey). '))
Anti-c-Jun Antibody, clone 6E4.4
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-c-Jun Antibody, clone 6E4.4 | 05-1076
Promotional Text:
Specificity:
This antibody detects c-Jun, regardless of phosphorylation state.
Molecular Weight:
~36-43 kDa.
Multiple bands may be visible, indicating various phosphorylation states.
Multiple bands may be visible, indicating various phosphorylation states.
Immunogen:
GST-tagged recombinant protein corresponding to c-Jun.
Clone:
6E4.4
Isotype:
IgG1κ
Background Information:
c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Demonstrated to react with human, mouse and rat.
Application Notes:
Western Blot Analysis (SNAP ID Technique): 5 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate using the SNAP ID Tecnique).
Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2).
Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2).
Control:
NIH/3T3 cell lysate
Quality Assurance:
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable at 2-8°C for 1 year from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- EC 2.7.11.24
- JNK
- JNK-46
- JNK1
- JNK1A2
- JNK21B1/2
- OTTHUMP00000019552
- OTTHUMP00000019555
- OTTHUMP00000019556
- OTTHUMP00000019558
- PRKM8
- SAPK1
Alternate Names:
- JNK1 alpha protein kinase
- JNK1 beta protein kinase
- JUN N-terminal kinase
- Stress-activated protein kinase JNK1
- c-Jun N-terminal kinase 1
- mitogen-activated protein kinase 8
- mitogen-activated protein kinase 8 isoform JNK1 alpha1
- mitogen-activated protein kinase 8 isoform JNK1 beta2
- protein kinase JNK1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involvedin both translocations and deletions in human malignancies.
Key Applications:
- Western Blotting
- Immunocytochemistry
UniProt Summary:
FUNCTION: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1.
SUBUNIT STRUCTURE: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP. Forms a complex with MAPK8IP1 and RGNEF By similarity. Interacts with NFATC4.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1.
SUBUNIT STRUCTURE: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP. Forms a complex with MAPK8IP1 and RGNEF By similarity. Interacts with NFATC4.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Product Name:
Anti-c-Jun, clone 6E4.4
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse