BrasilInformações para Pedidos
- : 05-1425
- : 500 μL
- : Variable
Product Images
14-3-3 clone 8C3 staining on fibroadenoma, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was used at 1g/mL, using IH...
Western Blot Analysis:
Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-14-3-3 PROTEINS (1:500 di...
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Anti-14-3-3, 33 kDa isoforms, clone 8C3
Recommended Replacement for: MAB3053| Reatividade à Espécie | Principais aplicações | Hospedeiro | Formato | Tipo de Anticorpo |
|---|---|---|---|---|
| R, Sh, H | WB | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-14-3-3, 33 kDa isoforms, clone 8C3
Replaces:
MAB3053
Specificity:
Clone 8C3 is specific for the 33 kDa epsilon isoform of 14-3-3 proteins and does not cross react with the 30 kDa 14-3-3 isoforms
Molecular Weight:
33 kDa
Epitope:
a.a. 208-244
Immunogen:
Directed against a synthetic peptide corresponding to rat 14-3-3 epsilon, amino acids 208-244.
Clone:
8C3
Isotype:
IgG2a
Background Information:
Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.
Species Reactivity:
- Rat
- Sheep
- Human
Species Reactivity Note:
Reacts with human. Expected to react in rat and sheep.
Control:
Jurkat cell lysate
Quality Assurance:
Evaluated by Western Blot in Jurkat cell lysates.
Western Blot Analysis: 1:500 dilution of this lot detected 14-3-3 PROTEINS on 10 ug of Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected 14-3-3 PROTEINS on 10 ug of Jurkat lysates.
Purification Method:
Unpurified, ascites liquid.
Presentation:
Ascites. Liquid containing 0.1% sodium azide.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- YWHAE
- KCIP-1
- MDS
- 14-3-3E
- MDCR
- FLJ45465
Alternate Names:
- 14-3-3 epsilon
- 14-3-3 protein epsilon
- mitochondrial import stimulation factor L subunit
- protein kinase C inhibitor protein-1
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation
- protein, epsilon polypeptide
- tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P62258 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
SIZE: 255 amino acids; 29174 Da
SUBUNIT: Homodimer. Interacts with NDEL1 (By similarity). Interacts with HCV core protein.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P62258 ## Belongs to the 14-3-3 family.
SIZE: 255 amino acids; 29174 Da
SUBUNIT: Homodimer. Interacts with NDEL1 (By similarity). Interacts with HCV core protein.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P62258 ## Belongs to the 14-3-3 family.
Protein/Isoform Description:
The 14-3-3 proteins are a family (at least seven members in mammals) of linkers and adaptors that comprise as much as 1% of total cellular protein. The proteins serve many functions, binding to other cytoplasmic and nuclear proteins through phosphorylated serine or threonine residues in virtually all eukaryotic cells. These binding interactions frequently have regulatory significance in many key cellular processes such as cell cycle progression and apoptosis, cell adhesion, and cell morphology. Aberrations in expression of 14-3-3 isoform expression has been linked to several genetic disorders and to cancer.
Product Name:
Anti-14-3-3, 33 kDa isoforms, clone 8C3
Concentration:
Variable
Antibody Type:
Monoclonal Antibody
Qty/Pk:
500 μL
Format:
Ascites
Host:
Mouse