Product Images
WB: NIH 3T3 cell lysate probed with anti-Cortactin (1μg/ml)
Anti-Cortactin (p80/85) Antibody, clone 4F11 | 05-180
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, B, H, Ht, M, R | ICC, IHC, IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Cortactin (p80/85) Antibody, clone 4F11 | 05-180
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Cortactin.
Molecular Weight:
80 kDa and 85 kDa
Immunogen:
Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src
Clone:
4F11
Isotype:
IgG1
Background Information:
Cortactin is present as a p80 and p85 kDa protein in many cell types. The protein was first identified as being a major substrate of the tyrosine kinase pp60SRC. The main structural feature of the protein is that at the N-terminus there are five and a half 37-amino acid repeats, these appear to be responsible for the actin-binding. This repeat region occupies about a third of the protein. The repeat region is followed by a helical region and then a proline rich region and finally at the C-terminus, an SH3 domain. The proline rich domain is thought to bind the SH2 domain of v-src the viral version of the normal c-src known to phosphorylate cortactin. Cortactin bundles microfilaments and this bundling is inhibited by tyrosine phosphorylation. One pathway that activates the pathway leading to tyrosine phosphorylation of cortactin results from a cell adhesion molecule in brain micro-vessel endothelial cells, another is the growth factor, but presumably the very many mechanisms for pp60src activation would also result in cortactin phosphorylation and subsequent loss of actin binding.
Species Reactivity:
- Avian
- Bovine
- Human
- Hamster
- Mouse
- Rat
Control:
Positive Control Included: 3T3 Cell Lysate (12-305) Other controls: PC-12 cell lysate, HeLa whole cell lysate or A-431 whole cell lysate
Quality Assurance:
routinely evaluated in mouse 3T3/A31 cell lysates or chicken fibroblast src-transformed cell lysate
Purification Method:
Protein G purfied
Presentation:
Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in Purified immunoglobulin in 10mM PBS, pH 7.4 with 0.05% sodium azide.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CTTN
- EMS1
- Amplaxin
- FLJ34459
- 1110020L01Rik
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
UniProt Summary:
FUNCTION: SwissProt: Q14247 # May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
SIZE: 550 amino acids; 61636 Da
SUBUNIT: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 (By similarity). Interacts with PLXDC2.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, ruffle (By similarity). Note=Associated with membrane ruffles and lamellipodia (By similarity).
SIMILARITY: SwissProt: Q14247 ## Contains 7 cortactin repeats. & Contains 1 SH3 domain.
SIZE: 550 amino acids; 61636 Da
SUBUNIT: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 (By similarity). Interacts with PLXDC2.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, ruffle (By similarity). Note=Associated with membrane ruffles and lamellipodia (By similarity).
SIMILARITY: SwissProt: Q14247 ## Contains 7 cortactin repeats. & Contains 1 SH3 domain.
Brand Family:
Upstate
Product Name:
Anti-Cortactin (p80/85) Antibody, clone 4F11 | 05-180
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 μg
Format:
Purified
Host:
Mouse