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IP: 3T3 cell lysate incubated with anti-FAK (7μg), then probed with anti-FAK polyclonal (06-543).
Anti-FAK, clone 2A7
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, H, M, R | IC, IP | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in incr
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Entrez Gene Number:
Entrez Gene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain
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Description:
Anti-FAK, clone 2A7
Clone:
2A7
Specificity:
Recognizes FAK.
Key Applications:
- Immunocytochemistry
- Immunoprecipitation
Application Notes:
Not recommended in Western Blot
Species Reactivity:
- Avian
- Human
- Mouse
- Rat
Isotype:
IgG1
Immunogen:
Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing p125FAK.
Molecular Weight:
125 kDa
Control:
Expressed in most tissue, 3T3/A31 cell lysate
Presentation:
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in Purified immunoglobulin in 10mM PBS, pH 7.4 containing no preservatives.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- PTK2
- pp125FAK
- FAK
- FADK
- FAK1
Quality Assurance:
routinely evaluated by immunoprecipitation of FAK from a mouse 3T3/A31 RIPA cell lysate, subsequently immunobloted with Anti-FAK (Catalog #06-543)
Antibody Category:
Signaling
Antibody Sub-Category:
Cytoskeletal Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein G Purified