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Anti-Nitrotyrosine, clone 1A6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | IC, IH, IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Nitrotyrosine, clone 1A6
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes nitrated protein.
Molecular Weight:
Predicted MW for nitrated-BSA: 66 kDa
Immunogen:
Nitrated KLH
Clone:
1A6
Isotype:
IgG2bκ
Background Information:
Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others
Species Reactivity:
Vertebrates
Control:
Immunohistochemistry: Human Alzheimer disease brain sections (Methacarn fixed), mouse liver
Immunoblotting: Nitrated-BSA
Indirect ELISA: nitrated-BSA
Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine
Immunoblotting: Nitrated-BSA
Indirect ELISA: nitrated-BSA
Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine
Quality Assurance:
routinely evaluated on peroxynitrite-treated A431 cells; no reaction was noted with non-nitrated proteins
Purification Method:
Protein G purfied
Presentation:
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Qty/Pk:
100 μg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-Nitrotyrosine, clone 1A6
Antibody Type:
Monoclonal Antibody