Anti-Fas (human, neutralizing),
clone ZB4

Anti-Fas (human, neutralizing),
clone ZB4

Upstate (Millipore)

This product does not induce apoptosis in cell culture.

This antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.

45 kDa

Recombinant human Fas.

clone ZB4

IgG1

Fas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.

Human

Human. This antibody does not recognize TNF and mouse Fas.

Flow Cytometry: A previous lot of this antibody used 5-20 μg/mL. Western Blot: A previous lot of this antibody used 5 μg/mL.

Various human cells

Evaluated by neutralization of apoptosis induced by anti-Fas (human, activating), clone CH11 (Catalog # 05-201) treatment of human Jurkat cells.

Neutralization: 10-500 ng/mL of this antibody was incubated for one hour with human Fas-transfected cells and inhibited the anti-Fas (human, activating), clone CH11 (Catalog # 05-201). Cell viability was assessed using a WST-1 assay

Protein A purfied

Ammonium sulfate and purified mouse monoclonal IgG1 in PBS containing 50% glycerol. Store at -20°C.

Stable for 1 year at from date of receipt.

P25445

NM_152877

• FAS
• OTTHUMP00000059646
• LFG
• FASTM
• ALPS1A
• TNFRSF6
• CD95
• FAS1
• FAIM2
• APT1
• NMP35
• APO-1

• APO-1 cell surface antigen
• Apo-1 antigen
• Apoptosis-mediating surface antigen FAS
• CD95 antigen
• FASLG receptor
• Fas (TNF receptor superfamily, member 6)
• Fas AMA
• Fas antigen
• apoptosis antigen 1
• tumor necrosis factor receptor superfamily member 6
• tumor necrosis factor receptor superfamily, member 6

This product is not available for sale in Japan.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

• Western Blotting
• Flow Cytometry
• Neutralizing

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.

FUNCTION: SwissProt: P25445 # Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
SIZE: 335 amino acids; 37732 Da
SUBUNIT: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. & Isoform 6: Secreted.
TISSUE SPECIFICITY: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
DOMAIN: SwissProt: P25445 Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
DISEASE: SwissProt: P25445 # Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
SIMILARITY: Contains 1 death domain. & Contains 3 TNFR-Cys repeats.

Upstate

Anti-Fas (human, neutralizing),
clone ZB4

1 mg/mL

Monoclonal Antibody

100 μg

Purified

Mouse