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WB: Rat brain microsomal preparation was probed with anti-MAP2 (0.5 μg/mL).
Anti-MAP2, clone 5F9
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Rb | WB, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-MAP2, clone 5F9
Trade Name:
Upstate (Millipore)
Specificity:
MAP2
Molecular Weight:
300kDa
Immunogen:
Purified rat brain microtubules MAP2
Isotype:
IgG1
Species Reactivity:
- Human
- Mouse
- Rat
- Rabbit
Quality Assurance:
routinely evaluated on rat brain microsomal preparation (Catalog #12-144).
Purification Method:
Protein G chromatography
Presentation:
200 µg of protein G purified mouse IgG1 in 200 µL of storage buffer (0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. Frozen at -20°C.
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CDKN1A
- MDA-6
- SDI1
- MDA6
- p21
- p21CIP1
- PIC1
- CIP1
- P21
- CDKN1
- WAF1
- CAP20
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
Key Applications:
- Western Blotting
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: P38936 # May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.
SIZE: 164 amino acids; 18119 Da
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Expressed in all adult human tissues, with 5- fold lower levels observed in the brain.
PTM: Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.
SIMILARITY: SwissProt: P38936 ## Belongs to the CDI family.
SIZE: 164 amino acids; 18119 Da
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Expressed in all adult human tissues, with 5- fold lower levels observed in the brain.
PTM: Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.
SIMILARITY: SwissProt: P38936 ## Belongs to the CDI family.
Qty/Pk:
200 µg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-MAP2, clone 5F9
Antibody Type:
Monoclonal Antibody