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WB: HeLa cell lysate probed with anti-Clusterin α chain (0.5μg/ml).
Anti-Clusterin α chain (human), clone 41D
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IC, IH, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Clusterin α chain (human), clone 41D
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes human clusterin α chain, Mr 40 kDa, and precursor protein.
Molecular Weight:
40 kDa
Immunogen:
Native clusterin purified from human serum. Clone 41D.
Clone:
41D
Isotype:
IgG1κ
Background Information:
Clusterin is also known as apolipoprotein J, complement lysis inhibitor, gp80, glycoprotein III, SGP-2, SP 40-40, TRPM2 and T64. It is a ubiquitous multifunctional protein of 80kD comprising of two disulfide-linked subunits, alpha and beta, and capable of interacting with a broad range of molecules. It is implicated in numerous biological processes including sperm maturation, lipid transport, regulation of the complement cascade, membrane recycling, cell death, immune regulation, cell adhesion and morphological transformation. In pathological conditions, it is an amyloid associated protein co-localising with fibrillar deposits in systemic and localised amyloid disorders. In Alzheimer ’s disease, clusterin is present in amyloid plaques and cerebrovascular deposits. In breast cancers, clusterin expression was found to correlate with tumour size. Clusterin is also implicated with endometrial cancers, myocardial infarction, renal diseases and atherosclerosis.
Species Reactivity:
Human
Species Reactivity Note:
Human.
Application Notes:
Immunohistochemistry:
Previous lots of this antibody at 5 µg/mL detected clusterin in paraffin embedded human brain sections.
Previous lots of this antibody at 5 µg/mL detected clusterin in paraffin embedded human brain sections.
Control:
HeLa cell lysate.
Quality Assurance:
Routinely evaluated on RIPA lysates from HeLa cells or MCF-7 cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of a previous lot detected human clusterin α chain in RIPA lysates from HeLa cells; previous lots detected the protein in human serum and MCF-7 cell lysates
Western Blot Analysis:
0.5-2 µg/mL of a previous lot detected human clusterin α chain in RIPA lysates from HeLa cells; previous lots detected the protein in human serum and MCF-7 cell lysates
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide. Frozen at -20°C.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CLU
- SP-40
- MGC24903
- NA1/NA2
- SGP-2
- AAG4
- KUB1
- APOJ
- CLI
- TRPM-2
- TRPM2
- Apo-J
- SGP2
Alternate Names:
- Apolipoprotein J
- Complement cytolysis inhibitor
- Complement-associated protein SP-40,40
- Ku70-binding protein 1
- Testosterone-repressed prostate message 2
- aging-associated protein 4
- clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)
- complement lysis inhibitor
- complement-associated protein SP-40
- sulfated glycoprotein 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P10909 # Not yet clear. It is known to be expressed in a variety of tissues and it seems to be able to bind to cells, membranes and hydrophobic proteins. It has been associated with programmed cell death (apoptosis).
SIZE: 449 amino acids; 52495 Da
SUBUNIT: Antiparallel disulfide-linked heterodimer. Interacts with CLUAP1.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P10909 ## Belongs to the clusterin family.
SIZE: 449 amino acids; 52495 Da
SUBUNIT: Antiparallel disulfide-linked heterodimer. Interacts with CLUAP1.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P10909 ## Belongs to the clusterin family.
Brand Family:
Upstate
Product Name:
Anti-Clusterin α chain (human), clone 41D
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse