Ordering Information
- : 05-368
- : 200 μg
- : 1 mg/mL
Product Images
WB: 20 micrograms of non-treated HeLa (lane 1) and colcemid-treated HeLa (lane 2) cell lysates were probed with 2µg/ml Anti-phospho-Ser/Thr-Pro, MPM-2...
WB: Non-treated HeLa (lane 1) and colcemid-treated HeLa (lane 2) cell lysates were probed with anti-phospho Ser/Thr-Pro, MPM-2 (2μg/ml).
see more > and colcemid-treated HeLa (lane 2) cell lysates were probed with 2µg/ml Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2).'))
 and colcemid-treated HeLa (lane 2) cell lysates were probed with anti-phospho Ser/Thr-Pro, MPM-2 (2μg/ml).'))
: Flow Cytometry analysis using anti-phospho-Ser/Thr-Pro, MPM-2). HeLa cells were treated with colcemid (green), or untreated (grey). '))
Anti-phospho-Ser/Thr-Pro MPM-2 Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | ELISA, IHC, IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-phospho-Ser/Thr-Pro Antibody, MPM-2 (Mitotic protein #2) | 05-368
Promotional Text:
Trade Name:
Upstate (Millipore)
Product Overview:
Specificity:
Recognizes phosphorylated serines or threonines when followed by a proline.
Molecular Weight:
varies depending on the protein you are detecting
Immunogen:
Mitotic human HeLa cell cytosolic lysate
Modifications:
Phosphorylation
Clone:
MPM-2
Isotype:
IgG1
Background Information:
Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
Species Reactivity:
Vertebrates
Control:
Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde
Quality Assurance:
routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
Purification Method:
Protein G purfied
Presentation:
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Brand Family:
Upstate
Product Name:
Anti-phospho-Ser/Thr-Pro Antibody, MPM-2 (Mitotic protein #2) | 05-368
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 μg
Format:
Purified
Host:
Mouse