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Ordering Information
- : 05-368MG
- : 1 mg
Product Images
WB: Non-treated HeLa (lane 1) and colcemid-treated HeLa (lane 2) cell lysates were probed with anti-phospho Ser/Thr-Pro, MPM-2 (2μg/ml).
WB: 20 micrograms of non-treated HeLa (lane 1) and colcemid-treated HeLa (lane 2) cell lysates were probed with 2µg/ml Anti-phospho-Ser/Thr-Pro, MPM-2...
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Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | ELISA, IH, IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2)
Trade Name:
Upstate (Millipore)
Product Overview:
Specificity:
Recognizes phosphorylated serines or threonines when followed by a proline.
Molecular Weight:
varies depending on the protein detected
Immunogen:
Mitotic human HeLa cell cytosolic lysate
Modifications:
Phosphorylation
Clone:
MPM-2
Isotype:
IgG1
Background Information:
Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
Species Reactivity:
Vertebrates
Control:
Colcemid-treated HeLa (lane 2) cell lysates, A431 mitotic cells fixed with 3.7% paraformaldehyde
Quality Assurance:
routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
Purification Method:
Protein G purfied
Presentation:
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in mouse IgG in 1.0 mL PBS containing no preservatives.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Brand Family:
Upstate
Protein/Isoform Description:
MPM-2 (mitotic protein monoclonal-2) is known industry wide as one of the most versatile phospho-serine/threonine antibodies available because of its ability to recognize more than 40 mitotic phosphoproteins. Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). Mitotic Protein Monoclonal 2 is an antibody which recognizes a phospho-epitope present in a large number of proteins, primarily during G2 and M phases of the cell cycle. While no true pan-phospho-serine or -threonine antibody has ever been produced, MPM-2 recognizes a wide variety of sites, most proline-directed, and is therefore suitable for the non-radioactive detection of many phospho-serines and -threonines.
Product Name:
Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2)
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 mg
Format:
Purified
Host:
Mouse