Ordering Information
- : 05-368MG
- : 1 mg
Product Images
WB: Non-treated HeLa (lane 1) and colcemid-treated HeLa (lane 2) cell lysates were probed with anti-phospho Ser/Thr-Pro, MPM-2 (2μg/ml).
WB: 20 micrograms of non-treated HeLa (lane 1) and colcemid-treated HeLa (lane 2) cell lysates were probed with 2µg/ml Anti-phospho-Ser/Thr-Pro, MPM-2...
see more > and colcemid-treated HeLa (lane 2) cell lysates were probed with anti-phospho Ser/Thr-Pro, MPM-2 (2μg/ml).'))
 and colcemid-treated HeLa (lane 2) cell lysates were probed with 2µg/ml Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2).'))
 labeling phospho-proteins in metaphase cells. The spindle, centrosomes and centromeric regions are stained. DNA is counterstained with propidium iodide (red). '))
Anti-phospho-Ser/Thr-Pro MPM-2 Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | ELISA, IHC, IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-phospho-Ser/Thr-Pro Antibody, MPM-2 (Mitotic protein #2) | 05-368MG
Promotional Text:
Trade Name:
Upstate (Millipore)
Product Overview:
Specificity:
Recognizes phosphorylated serines or threonines when followed by a proline.
Molecular Weight:
varies depending on the protein detected
Immunogen:
Mitotic human HeLa cell cytosolic lysate
Modifications:
Phosphorylation
Clone:
MPM-2
Isotype:
IgG1
Background Information:
Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
Species Reactivity:
Vertebrates
Control:
Colcemid-treated HeLa (lane 2) cell lysates, A431 mitotic cells fixed with 3.7% paraformaldehyde
Quality Assurance:
routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
Purification Method:
Protein G purfied
Presentation:
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in mouse IgG in 1.0 mL PBS containing no preservatives.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Brand Family:
Upstate
Product Name:
Anti-phospho-Ser/Thr-Pro Antibody, MPM-2 (Mitotic protein #2) | 05-368MG
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 mg
Format:
Purified
Host:
Mouse