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WB: Jurkat cell lysate probed with anti-FADD at 1μg/ml
Anti-FADD, clone 1F7
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-FADD, clone 1F7
Trade Name:
Upstate (Millipore)
Specificity:
FADD
Molecular Weight:
28kDa
Immunogen:
GST-tagged full-length fusion protein corresponding to human FADD
Isotype:
IgG1
Species Reactivity:
- Human
- Mouse
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Purification Method:
Protein A chromatography
Presentation:
PBS, pH 7.2 and 50% glycerol
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- FADD
- GIG3
- MGC8528
- MORT1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q13158 # Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
SIZE: 208 amino acids; 23279 Da
SUBUNIT: Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and LRDD.
TISSUE SPECIFICITY: Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
DOMAIN:SwissProt: Q13158 Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
PTM: Phosphorylated.
SIMILARITY: Contains 1 death domain. & Contains 1 DED (death effector) domain.
SIZE: 208 amino acids; 23279 Da
SUBUNIT: Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and LRDD.
TISSUE SPECIFICITY: Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
DOMAIN:SwissProt: Q13158 Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
PTM: Phosphorylated.
SIMILARITY: Contains 1 death domain. & Contains 1 DED (death effector) domain.
Qty/Pk:
100 µg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-FADD, clone 1F7
Antibody Type:
Monoclonal Antibody