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WB: HeLa cell nuclear extract probed with anti-Rad51 at 0.5μg/ml
Anti-Rad51, clone 3C10
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Rad51, clone 3C10
Trade Name:
Upstate (Millipore)
Specificity:
Rad51; slight cross-reactivity with DMC1
Molecular Weight:
37kDa
Immunogen:
Full length human Rad51
Isotype:
IgG1
Background Information:
Rad51 is similar to RecA, both biochemically and structurally. It promotes homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57, and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD51D). Recent studies suggested that Rad51 is involved not only in homologous recombination but also in cell proliferation regulation.
Species Reactivity:
- Human
- Mouse
- Rat
Control:
HeLa cell nuclear extract
Quality Assurance:
routinely evaluated by immunoblot on HeLa nuclear lysate
Purification Method:
Protein G chromatography
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- RAD51
- RAD51L3
- hRAD51
- HRAD51
- RECA
- RAD51D
- RAD51A
- HsT16930
- BRCC5
- HsRad51
- HsRAD51
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.
Key Applications:
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q06609 # May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments.
SIZE: 339 amino acids; 36966 Da
SUBUNIT: Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1.
SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage.
TISSUE SPECIFICITY: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
PTM: Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.DISEASE:SwissProt: Q06609 # Defects in RAD51 are associated with breast cancer (BC) [MIM:114480].
SIMILARITY: SwissProt: Q06609 ## Belongs to the recA family. RAD51 subfamily. & Contains 1 HhH domain.
SIZE: 339 amino acids; 36966 Da
SUBUNIT: Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1.
SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage.
TISSUE SPECIFICITY: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
PTM: Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.DISEASE:SwissProt: Q06609 # Defects in RAD51 are associated with breast cancer (BC) [MIM:114480].
SIMILARITY: SwissProt: Q06609 ## Belongs to the recA family. RAD51 subfamily. & Contains 1 HhH domain.
Qty/Pk:
200 µg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-Rad51, clone 3C10
Antibody Type:
Monoclonal Antibody