BrasilInformações para Pedidos
- : 05-538
- : 200 μg
- : 1 mg/mL
Product Images
WB: 3T3 cell lysate (lane 1) and 3T3+PDGF cell lysate (lane 2) was probed with anti-phospho-Raf-1 (Ser338) (2μg/ml).
Anti-phospho-Raf-1 (Ser338)
| Reatividade à Espécie | Principais aplicações | Hospedeiro | Formato | Tipo de Anticorpo |
|---|---|---|---|---|
| M | WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-phospho-Raf-1 (Ser338)
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Raf-1 phosphorylated at Serine 338.
Molecular Weight:
74 kDa
Immunogen:
Peptide corresponding to residues 333-345 of human Raf-1
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Mouse
Control:
Expressed in most tissue, lysates from 3T3/NIH cells stimulated with PDGF
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells stimulated with PDGF
Purification Method:
Protein G purfied
Presentation:
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- RAF1
- cRaf
- Raf-1
- c-Raf
- RAF
- CRAF
- C-RAF
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
UniProt Summary:
FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Brand Family:
Upstate
Product Name:
Anti-phospho-Raf-1 (Ser338)
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 μg
Format:
Purified
Host:
Mouse