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Immagini del prodotto
IP/WB: MCF-7 cell lysate was incubated with Anti-Ec/EphA2, clone B2D6, then probed with no primary antibody (lane 1) or anti-Eck/EphA2, clone D7 (lane...
Anti-Eck/EphA2, clone B2D6
| Specie riconosciute | Applicazioni fondamentali | Ospite | Forma | Tipo di anticorpo |
|---|---|---|---|---|
| H | IP | Mouse | Ascites | Monoclonal Antibody |
Numero UniProt:
UniProt Summary
FUNCTION: SwissProt: P99999 # Plays a role in apoptosis. Suppression of the anti- apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
SIZE: 105 amino acids; 11749 Da
SUBCELLULAR LOCATION: Mitochondr ... visualizza altro »
SIZE: 105 amino acids; 11749 Da
SUBCELLULAR LOCATION: Mitochondr ... visualizza altro »
Numero GenBank:
Entrez Gene Summary
This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the h
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Description:
Anti-Eck/EphA2, clone B2D6
Clone:
B2D6
Specificity:
Recognizes Eck/EphA2/Mpk-5.
Key Applications:
Immunoprecipitation
Application Notes:
Not recommended for WB.
Species Reactivity:
Human
Isotype:
IgG
Immunogen:
Native Epithelial Cell Kinase (Eck, also referred to as EphA2 and Mpk-5), isolated by purification of phosphotyrosine-containing proteins from ras-transformed human breast epithelial cells
Molecular Weight:
140 kDa
Control:
Expressed in most tissue, MCF-7 cell lysate
Presentation:
Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- CYCS
- CYC
- HCS
Quality Assurance:
routinely evaluated by immunoprecipitation of EphA2 from MCF-7 RIPA lysate, subsequent immunoblotting performed using Anti-Eck/EphA2, clone D7 antibody (Catalog #05-480)
Antibody Category:
Neuroscience
Antibody Sub-Category:
Growth Cones & Axon Guidance
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Asc
Concentration:
Variable
Antibody Type:
Monoclonal Antibody
Format:
Ascites
Host:
Mouse
Purification Method:
UnPurified