Content Loading...
Content Loading...
Product Images
IP/WB: MCF-7 cell lysate was incubated with Anti-Ec/EphA2, clone B2D6, then probed with no primary antibody (lane 1) or anti-Eck/EphA2, clone D7 (lane...
Anti-Eck/EphA2, clone B2D6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-Eck/EphA2, clone B2D6
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Eck/EphA2/Mpk-5.
Molecular Weight:
140 kDa
Immunogen:
Native Epithelial Cell Kinase (Eck, also referred to as EphA2 and Mpk-5), isolated by purification of phosphotyrosine-containing proteins from ras-transformed human breast epithelial cells
Clone:
B2D6
Isotype:
IgG
Species Reactivity:
Human
Application Notes:
Not recommended for WB.
Control:
Expressed in most tissue, MCF-7 cell lysate
Quality Assurance:
routinely evaluated by immunoprecipitation of EphA2 from MCF-7 RIPA lysate, subsequent immunoblotting performed using Anti-Eck/EphA2, clone D7 antibody (Catalog #05-480)
Purification Method:
Unpurified
Presentation:
Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CYCS
- CYC
- HCS
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome.
Key Applications:
Immunoprecipitation
UniProt Summary:
FUNCTION: SwissProt: P99999 # Plays a role in apoptosis. Suppression of the anti- apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
SIZE: 105 amino acids; 11749 Da
SUBCELLULAR LOCATION: Mitochondrion matrix.
PTM: Binds 1 heme group per subunit.
SIMILARITY: SwissProt: P99999 ## Belongs to the cytochrome c family.
SIZE: 105 amino acids; 11749 Da
SUBCELLULAR LOCATION: Mitochondrion matrix.
PTM: Binds 1 heme group per subunit.
SIMILARITY: SwissProt: P99999 ## Belongs to the cytochrome c family.
Qty/Pk:
100 μL
Brand Family:
Upstate
Format:
Ascites
Host:
Mouse
Product Name:
Anti-Eck/EphA2, clone B2D6
Concentration:
Variable
Antibody Type:
Monoclonal Antibody