Anti-Caspase 7, clone 7-1-11

P55210

FUNCTION: SwissProt: P55210 # Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly- 217' bond. Overexpression promotes programmed cell death.
SIZE: 303 amino acids; 34277 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
PTM: Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
SIMILARITY: SwissProt: P55210 ## Belongs to the peptidase C14 family. ... hide » 更多信息 »

NM_033339

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. ... hide » 更多信息 »

Anti-Caspase 7, clone 7-1-11

Caspase 7 and its cleaved isoform

• Immunoprecipitation
• Western Blotting

Human

IgG

GST fusion protein corresponding to residues 1-303 of human Caspase 7

37kDa (cleaved form at 19kDa)

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

2 years at -20°C

• CASP7
• CASP-7
• ICE-LAP3
• CMH-1
• MCH3

routinely evaluated by immunoblot on RIPA lysates from Jurkat cells

• Apoptosis & Cancer
• Metabolism

• Caspases
• Enzymes & Biochemistry

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Upstate (Millipore)

Monoclonal Antibody

Purified

Mouse

This antibody has been shown to detect the cleaved form of Caspase 7 in Kirch, D. G., et al., JBC 274: 21155, 1999

Protein G chromatography