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WB: non-transfected (lane1) or Herstatin/HER-2 transfected 293 cell lysates (lane 2) probed with anti-Herstatin (μg/ml).
Anti-Herstatin, clone CW001
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Herstatin, clone CW001
Trade Name:
Upstate (Millipore)
Qty/Pk:
200 µg
Key Applications:
- Western Blotting
- Immunohistochemistry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
UniProt Summary:
FUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
SIZE: 1255 amino acids; 137910 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
SIZE: 1255 amino acids; 137910 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
Molecular Weight:
Herstatin (60kDa) and Herstatin aggregates (150kDa)
Quality Assurance:
routinely evaluated by immunoblot using the Herstatin Detection Kit (Catalog #17-354). Previous lots have also been tested with HEK293 cells transfected with Herstatin/HER-2neu
Immunogen:
Recombinant human Herstatin corresponding to residues 340-418
Brand Family:
Upstate
Format:
Purified
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, before the addition of glycerol to 30%
UniProt Number:
Storage Conditions:
2 years at -20°C
Host:
Mouse
Isotype:
IgG
Gene Symbol:
- ERBB2
- HER2
- HER-2
- NGL
- herstatin
- erbB-2
- C-erbB-2
- NEU
- TKR1
- p185erbB2
- HER-2/neu
Product Name:
Anti-Herstatin, clone CW001
Entrez Gene Number:
Antibody Type:
Monoclonal Antibody
Purification Method:
Protein G chromatography
Specificity:
Herstatin and Herstatin aggregates