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WB: Non-stimulated (lane 1) and PMA-stimulated (lane 2) human red blood cell lysate, was probed with anti-phospho-Adducin, clone pADD (S724) (0.5μg/ml...
Anti-phospho-Adducin (Ser724), clone pADD
| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H, M, R | IP, WB, IC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-phospho-Adducin (Ser724), clone pADD
Trade Name:
Upstate (Millipore)
Specificity:
phospho-Adducin isoforms
Molecular Weight:
80-120kDa
Immunogen:
peptide (FRTP[pS]FLKK) corresponding to amino acids 720-728 of rat phospho-α-Adducin (Ser724)
Modifications:
Phosphorylation
Clone:
pADD
Isotype:
IgG
Species Reactivity:
- Human
- Mouse
- Rat
Quality Assurance:
routinely evaluated by immunoblot on lysates from human red blood cells stimulated with PMA
Purification Method:
Protein G chromatography
Presentation:
0.7M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ADD1
- ADDA
- Alpha-adducin
- MGC44427
- MGC3339
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Key Applications:
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
UniProt Summary:
FUNCTION: SwissProt: P35611 # Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
SIZE: 737 amino acids; 80955 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
TISSUE SPECIFICITY: Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
DOMAIN: SwissProt: P35611 Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
PTM: The N-terminus is blocked.
SIMILARITY: Belongs to the aldolase class II family. Adducin subfamily.
SIZE: 737 amino acids; 80955 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
TISSUE SPECIFICITY: Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
DOMAIN: SwissProt: P35611 Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
PTM: The N-terminus is blocked.
SIMILARITY: Belongs to the aldolase class II family. Adducin subfamily.
Qty/Pk:
100 µg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-phospho-Adducin (Ser724), clone pADD
Antibody Type:
Monoclonal Antibody