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Anti-Bmi-1, clone F6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Rb | IC, IP, WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P35226 # Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein
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Entrez Gene Number:
Description:
Anti-Bmi-1, clone F6
Background Information:
The Bmi1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors.
Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.
Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.
Alternate Names:
Polycomb group RING finger protein 4; RING finger protein 51
Clone:
F6
Specificity:
Recognizes Bmi-1 (triplet).
Key Applications:
- Immunocytochemistry
- Immunoprecipitation
- Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
- Rabbit
Isotype:
IgG1
Immunogen:
Immunized with recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.
Molecular Weight:
40-44 kDa
Control:
U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos
Presentation:
Immunoaffinity Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- BMI1
- MGC12685
- PCGF4
- flvi-2/bmi-1
- RNF51
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from U2OS cells.
Antibody Category:
Nuclear Function
Antibody Sub-Category:
Chromatin Biology
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format:
Purified
Host:
Mouse
Format Code:
Pur
Antibody Type:
Monoclonal Antibody
Purification Method:
Protein G Purified