Content Loading...
Content Loading...
Product Images
WB: HeLa nuclear extract was probed with Anti-BRCA2. Arrow indicates BRCA2 (390kDa).
Anti-BRCA2, clone 5.23
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-BRCA2, clone 5.23
Trade Name:
Upstate (Millipore)
Specificity:
BRCA2
Molecular Weight:
390kDa
Immunogen:
GST fusion protein to residues 2959-3418 of human BRCA2 protein
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot on in HeLa nuclear extract
Purification Method:
Protein G chromatography
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide.
Storage Conditions:
Store at 2-8°C. Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- BRCA2
- FACD
- FANCB
- FAD1
- FAD
- FANCD1
- BRCC2
- OTTHUMP00000042401
- FANCD
Sales Restrictions:
Due to license agreement restrictions, this product cannot be purchased for resale.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintainance of genome stability, specificallythe homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.
Key Applications:
- Western Blotting
- Immunocytochemistry
UniProt Summary:
FUNCTION: SwissProt: P51587 # Involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation.
SIZE: 3418 amino acids; 384225 Da
SUBUNIT: Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoints functions. Interacts with WDR16.
TISSUE SPECIFICITY: Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
PTM: Phosphorylated by ATM upon irradiation-induced DNA damage.
DISEASE: SwissProt: P51587 # Defects in BRCA2 are a cause of genetic susceptibility to breast cancer (BC) [MIM:600185, 114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Mutations in BRCA2 are thought to be responsible for some inherited breast cancer. It is linked with male breast cancer. & Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. Fanconi anemia [MIM:227650] is an autosomal recessive disorder affecting all bone marrow elements and associated with cardiac, renal, and limb malformations as well as dermal pigmentary changes.
SIMILARITY: SwissProt: P51587 ## Contains 8 BRCA2 repeats.
SIZE: 3418 amino acids; 384225 Da
SUBUNIT: Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoints functions. Interacts with WDR16.
TISSUE SPECIFICITY: Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
PTM: Phosphorylated by ATM upon irradiation-induced DNA damage.
DISEASE: SwissProt: P51587 # Defects in BRCA2 are a cause of genetic susceptibility to breast cancer (BC) [MIM:600185, 114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer. Mutations in BRCA2 are thought to be responsible for some inherited breast cancer. It is linked with male breast cancer. & Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. Fanconi anemia [MIM:227650] is an autosomal recessive disorder affecting all bone marrow elements and associated with cardiac, renal, and limb malformations as well as dermal pigmentary changes.
SIMILARITY: SwissProt: P51587 ## Contains 8 BRCA2 repeats.
Qty/Pk:
200 µg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-BRCA2, clone 5.23
Antibody Type:
Monoclonal Antibody