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WB: Non-treated (lane 1) or PDGF-treated (lane 2) mouse NIH-3T3 cell lysate was probed with anti-phospho-Akt1/PKBα (Ser473) (0.5μg/ml). Arrow indica...
Anti-phospho-Akt1/PKBα (Ser473), clone 11E6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H, Ca | ELISA, WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surfac
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Entrez Gene Number:
Entrez Gene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuro
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Description:
Anti-phospho-Akt1/PKBα (Ser473), clone 11E6
Background Information:
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.
Alternate Names:
- Protein kinase B
- RAC-alpha serine/threonine-protein kinase
- murine thymoma viral (v-akt) oncogene homolog-1
- rac protein kinase alpha
- v-akt murine thymoma viral oncogene homolog 1
Clone:
11E6
Specificity:
Recognizes phosphorylated Akt1/PKBα at Ser473, Mr 60 kDa. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.
Key Applications:
- ELISA
- Western Blotting
Application Notes:
ELISA:
A previous lot was recommended at 0.05 mg/mL by an independent laboratory for ELISA.
A previous lot was recommended at 0.05 mg/mL by an independent laboratory for ELISA.
Species Reactivity:
- Mouse
- Rat
- Human
- Canine
Species Reactivity Note:
Human, mouse, canine and rat.
Isotype:
IgG1κ
Immunogen:
KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.
Modifications:
Phosphorylation
Molecular Weight:
60 kDa
Control:
PDGF treated NIH-3T3 cell lysates.
Presentation:
Purified mouse monoclonal IgG1κ lyophilized from 1 mL 2X PBS, PEG, sucrose, and 0.09% sodium azide.
Reconstitute with 1 mL H2O (15 min. at RT). Concentration after reconstitution: 100 µg/mL
Reconstitute with 1 mL H2O (15 min. at RT). Concentration after reconstitution: 100 µg/mL
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- AKT1
- RAC
- PRKBA
- MGC99656
- RAC-ALPHA
- RAC-PK-alpha
- C-AKT
- PKB
- AKT
Quality Assurance:
Evaluated by western blot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 100 ng/mL PDGF for 20 minutes.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 100 ng/mL PDGF for 20 minutes.
Antibody Category:
Signaling
Antibody Sub-Category:
PI3K, Akt, & mTOR Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.