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WB: Mouse heart cytosolic lysate was probed with anti-phospho-Acetyl CoA Carboxylase (Ser79).
Anti-phospho-Acetyl CoA Carboxylase (Ser79), clone BK102
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-phospho-Acetyl CoA Carboxylase (Ser79), clone BK102
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated Acetyl CoA Carboxylase (Ser79).
Molecular Weight:
257 kDa
Immunogen:
Synthetic peptide corresponding to rat Acetyl CoA Carboxylase
Modifications:
Phosphorylation
Clone:
BK102
Isotype:
IgG1λ
Species Reactivity:
- Mouse
- Rat
Control:
Acid extracts of HeLa cells
Quality Assurance:
Routinely evaluated by immunoblot on HeLa acid extracts
Purification Method:
Protein G purfied
Presentation:
Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% sodium azide, pH 7.4.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ACACA
- ACC-alpha
- ACCA
- ACC
- ACC1
- ACAC
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q13085 # Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
COFACTOR: Biotin. & Binds 2 manganese ions per subunit.
SIZE: 2346 amino acids; 265554 Da
SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.
SIMILARITY: SwissProt: Q13085 ## Contains 1 ATP-grasp domain. & Contains 1 biotin carboxylation domain. & Contains 1 biotinyl-binding domain. & Contains 1 carboxyltransferase domain.
COFACTOR: Biotin. & Binds 2 manganese ions per subunit.
SIZE: 2346 amino acids; 265554 Da
SUBUNIT: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.
SIMILARITY: SwissProt: Q13085 ## Contains 1 ATP-grasp domain. & Contains 1 biotin carboxylation domain. & Contains 1 biotinyl-binding domain. & Contains 1 carboxyltransferase domain.
Qty/Pk:
200 μg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-phospho-Acetyl CoA Carboxylase (Ser79), clone BK102
Antibody Type:
Monoclonal Antibody