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WB: Src Immunoprecipitated with anti-Src (05-184) was either treated (lane 1) or untreated (lanes 2 and 3) with the phosphotyrosine phosphatase YOP (1...
Anti-phospho-Src (Tyr416), clone 9A6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IP | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase ... see more »
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Description:
Anti-phospho-Src (Tyr416), clone 9A6
Background Information:
Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
Alternate Names:
- proto-oncogene tyrosine-protein kinase SRC
- protooncogene SRC, Rous sarcoma
- tyrosine kinase pp60c-src
- tyrosine-protein kinase SRC-1
- v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
- v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Clone:
clone 9A6
Specificity:
Recognizes phospho-Src, Mr 60kDa. The antibody does not crossreact with the nonphosphorylated form of Src or with unrelated phosphorylation sites. Predicted to recognize all Src family members phosphorylated at the tyrosine corresponding to Tyr416 of avian Src.
Key Applications:
- Western Blotting
- Immunoprecipitation
Application Notes:
Immunoprecipitation/Immunoblot Analysis: 4 μg/mL of a previous lot detected phospho-Src immunoprecipitated from 3T3 cell lysates with 10 μg of anti-Src (Catalog # 05-184), but not after phosphatase treatment with YOP.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human, mouse, and rat.
Isotype:
IgG1κ
Immunogen:
KLH-conjugated, synthetic peptide containing phosphotyrosine in the sequence context corresponding to tyrosine 416 of avian Src. Clone 9A6.
Modifications:
Phosphorylation
Molecular Weight:
60 kDa
Control:
Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein
Presentation:
Purified mouse monoclonal IgG1κ lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 μL H2O (15 min, RT) for a final concentration of 1 mg/mL. Lyophilized. Store at -20ºC.
Rehydration: Rehydrate with 100 μL of sterile, distilled water to make a 1 mg/mL solution.
Rehydration: Rehydrate with 100 μL of sterile, distilled water to make a 1 mg/mL solution.
Storage Conditions:
Stable for 1 year at from date of receipt.
Gene Symbol:
- SRC
- c-src
- p60-Src
- SRC1
- c-SRC
- ASV
- pp60c-src
Quality Assurance:
Evaluated by immunoprecipitating Src from 3T3 cell lysates with anti-Src (Catalog #05-184), but not after phosphatase treatment with YOP.
Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase.
Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase.
Antibody Category:
Signaling
Antibody Sub-Category:
Cytoskeletal Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Product Overview:
Specific src isoform is unknown
Purification Method:
Thiophilic adsorption and size exclusion chromatography