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WB: HUVEC membrane cell lysate was probed with anti-Flt-1 (2μg/ml).
Anti-Flt-1, clone BK302
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Flt-1, clone BK302
Trade Name:
Upstate (Millipore)
Specificity:
Flt-1
Molecular Weight:
~180kDa
Immunogen:
GST fusion protein corresponding to residues 1251-1338 of human Flt-1
Isotype:
IgG1κ
Species Reactivity:
Human
Application Notes:
Not recommended for IP.
Quality Assurance:
routinely evaluated by immunoblot on Human umbilical vein endothelium (HUVEC) membrane preps
Purification Method:
Protein G purfied
Presentation:
of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- FLT1
- VEGFR1
- FLT
- FRT
- VEGFR-1
- Flt-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Oncogene FLT belongs to the src gene family and is related to oncogene ROS (MIM 165020). Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The sequence structure of the FLT gene resembles that of the FMS gene (MIM 164770); hence, Yoshida et al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.[supplied by OMIM]
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P17948 # Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
SIZE: 1338 amino acids; 150769 Da
SUBUNIT: Interacts in vitro with various phosphotyrosine-binding proteins, including PLC-gammas, PTPN11, GRB2, CRK and NCK1.
SUBCELLULAR LOCATION: Isoform Flt1: Cell membrane; Single-pass type I membrane protein. & Isoform sFlt1: Secreted.
TISSUE SPECIFICITY: Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
SIMILARITY: SwissProt: P17948 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
SIZE: 1338 amino acids; 150769 Da
SUBUNIT: Interacts in vitro with various phosphotyrosine-binding proteins, including PLC-gammas, PTPN11, GRB2, CRK and NCK1.
SUBCELLULAR LOCATION: Isoform Flt1: Cell membrane; Single-pass type I membrane protein. & Isoform sFlt1: Secreted.
TISSUE SPECIFICITY: Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
SIMILARITY: SwissProt: P17948 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Qty/Pk:
200 µg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-Flt-1, clone BK302
Antibody Type:
Monoclonal Antibody