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Ordering Information

  • : 05-716
  • : 200 µL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.
  • Product Family Information

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WB: C2C12 cell lysate was probed with anti-Myosin Heavy Chain (MyHC) (1:1000 dilution).

Anti-Myosin Heavy Chain Antibody, clone A4.1025

Species Reactivity Key Applications Host Format Antibody Type
H, R, M, Zebrafish, Dr, Rb IHC, WB Mouse Ascites Monoclonal Antibody
Description:
Anti-Myosin Heavy Chain Antibody, clone A4.1025
Promotional Text:
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes myosin heavy chain, Mr ~220 kDa. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products.
Molecular Weight:
~220 kDa
Immunogen:
Partially purified protein from adult human skeletal muscle myosin. Clone A4.1025.
Clone:
A4.1025
Isotype:
Background Information:
Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.
Species Reactivity:
  • Human
  • Rat
  • Mouse
  • Zebrafish
  • Drosophila
  • Rabbit
Species Reactivity Note:
Human, mouse, rat, rabbit, zebrafish and Drosophila.
Application Notes:
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde in a previous lot (Blagden, C.S., 1997; Cho, M., 1994).
Control:
RIPA lysates from C2C12 cells
Quality Assurance:
Routinely evaluated by Western Blot on RIPA lysates from C2C12 cells.

Western Blot Analysis:
1:1000-1:5000 dilution of this lot detected Myosin Heavy Chain (MyHC) in RIPA lysates from Rabbit skeletal muscle cells.
Purification Method:
Unpurified
Presentation:
Unpurified mouse ascites monoclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • MYH1
  • MyHC-2X/D
  • MGC133384
  • MYHa
  • MyHC-IIx/d
  • MyHC-2x
  • Myosin-1
  • MYHSA1
Alternate Names:
  • Myosin heavy chain 1
  • Myosin heavy chain 2x
  • Myosin heavy chain IIx/d
  • Myosin heavy chain, skeletal muscle, adult 1
  • myosin, heavy chain 1, skeletal muscle, adult
  • myosin, heavy polypeptide 1, skeletal muscle, adult
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunohistochemistry
  • Western Blotting
Entrez Gene Summary:
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
UniProt Summary:
FUNCTION: SwissProt: P12882 # Muscle contraction.
SIZE: 1939 amino acids; 223115 Da
SUBUNIT: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
DOMAIN: SwissProt: P12882 The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
SIMILARITY: Contains 1 IQ domain. & Contains 1 myosin head-like domain.
MISCELLANEOUS: Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Brand Family:
Upstate
Product Name:
Anti-Myosin Heavy Chain Antibody, clone A4.1025
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 µL
Format:
Ascites
Host:
Mouse
Applications:
Anti-Myosin Heavy Chain Antibody, clone A4.1025 is an antibody against Myosin Heavy Chain for use in IH & WB.

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