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WB: MEF1 cell lysate was probed with anti-PML, clone 36.1-104 (0.5μg/ml).
Anti-PML, clone 36.1-104
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IC, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-PML, clone 36.1-104
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes PML.
Molecular Weight:
90-106 kDa
Immunogen:
6His fusion protein corresponding to mouse PML (promyelocytic leukemia protein)
Clone:
36.1-104
Isotype:
IgG
Background Information:
Promyeloctic Leukemia Gene product (PML) is a nuclear protein with growth-suppressive properties originally identified in the context of the PML-retinoic receptor alpha (RAR alpha) fusion protein of acute promyelocytic leukemia. PML localizes within distinct nuclear structures, called nuclear bodies, which are disrupted by the expression of PML-RAR alpha.
Species Reactivity:
Mouse
Control:
MEF1 cell lysate
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from mouse embryo fibroblast (MEF1) cells
Purification Method:
Protein G purfied
Presentation:
Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PML
- PP8675
- RNF71
- TRIM19
- MYL
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
Key Applications:
- Immunocytochemistry
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P29590 # Probable transcription factor. May play an important role in recruitment of ELF4 into PML nuclear bodies.
SIZE: 882 amino acids; 97517 Da
SUBUNIT: Interacts with SIRT1, TOPBP1, TRIM27 and TRIM69. Interacts with the C-terminus of ELF4. Interacts with Lassa virus Z protein and rabies virus phosphoprotein (By similarity).
SUBCELLULAR LOCATION: Nucleus. Note=Localized to PML nuclear bodies.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
DISEASE: SwissProt: P29590 # A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
SIMILARITY: SwissProt: P29590 ## Contains 2 B box-type zinc fingers. & Contains 1 RING-type zinc finger.
SIZE: 882 amino acids; 97517 Da
SUBUNIT: Interacts with SIRT1, TOPBP1, TRIM27 and TRIM69. Interacts with the C-terminus of ELF4. Interacts with Lassa virus Z protein and rabies virus phosphoprotein (By similarity).
SUBCELLULAR LOCATION: Nucleus. Note=Localized to PML nuclear bodies.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
DISEASE: SwissProt: P29590 # A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
SIMILARITY: SwissProt: P29590 ## Contains 2 B box-type zinc fingers. & Contains 1 RING-type zinc finger.
Qty/Pk:
200 μg
Brand Family:
Upstate
Format:
Purified
Host:
Mouse
Product Name:
Anti-PML, clone 36.1-104
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody