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Ordering Information
- : 05-724
- : 200 μg
- : 1 mg/mL
Product Images
WB: 0.5-2.0μg/ml of this lot detected Myc-tagged recombinant protein in sequence contexts (lane 5) not well recognized by anti-MycTag, clone 9E10...
Anti-Myc Tag, clone 4A6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | Chromatin Immunoprecipitation (ChIP), IC, IF, IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Myc Tag, clone 4A6
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc.
Molecular Weight:
Predicted MW for human Myc: 49 kDa
Immunogen:
peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc
Clone:
4A6
Isotype:
IgG
Background Information:
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
Species Reactivity:
Human
Control:
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Quality Assurance:
routinely evaluated by immunoblot on Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag, clone 9E10 (Catalo
Purification Method:
Protein G purfied
Presentation:
Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
Storage Conditions:
Maintain at 2 to 8°C for 1 year from date of shipment.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MYC
- C-MYC
- c-Myc
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Immunocytochemistry
- Immunofluorescence
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
UniProt Summary:
FUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
SIZE: 439 amino acids; 48804 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC.
DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
SIZE: 439 amino acids; 48804 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated by PRKDC.
DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
Brand Family:
Upstate
Product Name:
Anti-Myc Tag, clone 4A6
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 μg
Format:
Purified
Host:
Mouse