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WB: 0.5-2.0μg/ml of this lot detected Myc-tagged recombinant protein in sequence contexts (lane 5) not well recognized by anti-MycTag, clone 9E10...
Anti-Myc Tag, clone 4A6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | Chromatin Immunoprecipitation (ChIP), IC, IF, IP, WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
SIZE: 439 amino acids; 48804 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts ... see more »
SIZE: 439 amino acids; 48804 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation ini
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Description:
Anti-Myc Tag, clone 4A6
Background Information:
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
Clone:
4A6
Specificity:
Recognizes recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Immunocytochemistry
- Immunofluorescence
- Immunoprecipitation
- Western Blotting
Species Reactivity:
Human
Isotype:
IgG
Immunogen:
peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc
Molecular Weight:
Predicted MW for human Myc: 49 kDa
Control:
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Presentation:
Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- MYC
- C-MYC
- c-Myc
Quality Assurance:
routinely evaluated by immunoblot on Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag, clone 9E10 (Catalo
Antibody Category:
Epitope Tags & General Use
Antibody Sub-Category:
Epitope Tags
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein G Purified