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ICC/IF: HeLa cells with or without myc-tagged Scc1 were used for indirect immunofluorescence. Cells were fixed with paraformaldehyde, and were then in...
WB: 0.5-2.0μg/ml of this lot detected Myc-tagged recombinant protein in sequence contexts (lane 5) not well recognized by anti-MycTag, clone 9E10...
Anti-Myc Tag, clone 4A6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | Chromatin Immunoprecipitation (ChIP), IF, IP, WB, IC | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
SIZE: 439 amino acids; 48804 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts ... see more »
SIZE: 439 amino acids; 48804 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation ini
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Description:
Anti-Myc Tag, clone 4A6
Specificity:
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc.
Key Applications:
- Chromatin Immunoprecipitation (ChIP)
- Immunofluorescence
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
Species Reactivity:
Human
Isotype:
IgG1
Immunogen:
peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc
Molecular Weight:
dependent upon the molecular weight of the tagged protein
Presentation:
PBS
Storage Conditions:
2 years at -20°C
Gene Symbol:
- MYC
- C-MYC
- c-Myc
Quality Assurance:
routinely evaluated by immunoblot on Myc-tagged recombinant protein in sequence contexts
Antibody Category:
Epitope Tags & General Use
Antibody Sub-Category:
Epitope Tags
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Product Overview:
Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.
Purification Method:
protein G purified